supported in part by National Basic Research Program of China (973 program; 2013CB943103);the National Natural Science Foundation of China (Grant No. 31072029, No.31272439, No. C170104 and No. 31230048);Ph.D. Programs Foundation of Ministry of Education of China (Grant No.20130204110017) for W. Zeng and W Dong;the scholarship from China Scholarship Council (CSC)
Background: Male germline stem cells(MGSCs) are a subpopulation of germ cells in the testis tissue. MGSCs are capable of differentiation into spermatozoa and thus are perfect targets for genomic manipulation to gen...