国家重点基础研究发展计划(2011CB100203)

作品数:4被引量:164H指数:4
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相关作者:王慧娜马兴亮初志战李日清刘耀光更多>>
相关机构:华南农业大学更多>>
相关期刊:《Journal of Integrative Plant Biology》《作物学报》《Molecular Plant》《Cell Research》更多>>
相关主题:RICETGMSSMALL_RNAPHOTOPERIODPGMS更多>>
相关领域:农业科学生物学更多>>
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高通量PCR模板植物基因组DNA制备方法被引量:20
《作物学报》2013年第7期1200-1205,共6页王慧娜 初志战 马兴亮 李日清 刘耀光 
国家重点基础研究发展计划(973计划)项目(2011CB100203)资助
制备大量生物样品的模板DNA用于PCR检测是费时费工的工作。本文介绍一种快速高通量的植物基因组DNA(gDNA)制备及其用于PCR基因型检测的操作方法。将一小段单子叶植物苗叶片(与96方孔板的孔深大致相同)或一小块(约2~5mg)双子叶植物叶片...
关键词:基因组DNA制备 PCR 基因分型 
A Novel Rice bHLH Transcription Factor, DTD, Acts Coordinately with TDR in Controlling Tapetum Function and Pollen Development被引量:21
《Molecular Plant》2013年第5期1715-1718,共4页Chonghui Ji Heying Li Libin Chen Min Xie Fengping Wang Yuanling Chen Yao-Guang Liu 
Dear Editor, Male reproductive development is an essential biological process for flowering plants and crucial for crop seed produc- tion. Formation of the male reproductive organ, the anther, involves a number of de...
Molecular Control of Male Reproductive Development and Pollen Fertility in Rice被引量:20
《Journal of Integrative Plant Biology》2012年第12期967-978,共12页Jing-Xin Guo Yao-Guang Liu 
supported by a grant from the National Basic Research Program of China(973 Program,2011CB100203)
Anther development and male fertility are essential biological pro- cesses for flowering plants and are important for crop seed produc- tion. Genetic manipulation of male fertility/sterility is critical for crop hybri...
关键词:Anther development cytoplasmic male sterility hybrid sterility male fertility MEIOSIS rice. 
Photoperiod- and thermo-sensitive genic male sterility in rice are caused by a point mutation in a novel noncoding RNA that produces a small RNA被引量:113
《Cell Research》2012年第4期649-660,共12页Hai Zhou Qinjian Liu Jing Li Dagang Jiang Lingyan Zhou Ping Wu Sen Lu Feng Li Liya Zhu Zhenlan Liu Letian Chen Yao-Guang Liu Chuxiong Zhuang 
Photoperiod- and thermo-sensitive genic male sterility (PGMS and TGMS) are the core components for hybrid breeding in crops. Hybrid rice based on the two-line system using PGMS and TGMS lines has been successfully d...
关键词:RICE PGMS TGMS noncoding RNA small RNA 
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