国家自然科学基金(30000032)

作品数:7被引量:42H指数:4
导出分析报告
相关作者:鲍锦库陈放吴传芳常丽青刘超更多>>
相关机构:四川大学中国科学院成都生物研究所成都军区联勤部更多>>
相关期刊:《Acta Biochimica et Biophysica Sinica》《Journal of Integrative Plant Biology》《应用与环境生物学报》《四川大学学报(自然科学版)》更多>>
相关主题:凝集素MOLECULAR_CLONING纯化红花石蒜抗病毒活性更多>>
相关领域:生物学医药卫生更多>>
-

检索结果分析

结果分析中...
条 记 录,以下是1-7
视图:
排序:
麦冬凝集素的氨基酸修饰和荧光光谱研究被引量:1
《四川大学学报(自然科学版)》2008年第6期1519-1526,共8页戴蕾 金辉 叶松华 王维 鲍锦库 
国家自然科学基金(30000032,30270331)
通过对麦冬凝集素(OJL)进行特殊氨基酸的化学修饰,揭示了OJL所含的6个Trp残基只有1个位于蛋白表面,Trp、Tyr和Ser/Thr不是OJL凝集活性所必需的氨基酸,而Arg是维持其活性的必需基团.OJL在天然状态下荧光发射峰在328 nm处,Trp周围的极性较...
关键词:麦冬凝集素(OJL) 化学修饰 荧光光谱 荧光淬灭 
Purification and Characterization of a Mannose-binding Lectin from the Rhizomes of Aspidistra elatior Blume with Antiproliferative Activity被引量:8
《Acta Biochimica et Biophysica Sinica》2007年第7期507-519,共13页Xiaochao XU Chuanfang WU Chao LIU Yongting LUO Jian LI Xinping ZHAO Els Van DAMME Jinku BAO 
This work was supported by the grants from the National Natural Science Foundation of China (30000032 and 30270331), the Science and Technology Fund for Distinguished Young Scholars of Sichuan Province (06ZQ026-035), the Key Technologies R&D Program of Sichuan Province (2006Z08-010) and European Commission (EMPR0) and the Centers of Excellence of the K. U. Leuven (EF/05/15)
A lectin with a novel N-terminal amino acid sequence was purified from the rhizomes of Aspidistra elatior Blume by ammonium sulphate precipitation, ion exchange chromatography on diethylaminoethyl-Sepharose and carbox...
关键词:antiproliferative activity Aspidistra elatior Blume chemical modification hemagglutinating activity mannose-binding lectin 
Purification, Biological Activities, and Molecular Cloning of a Novel Mannose-Binding Lectin from Bulbs of Zephyranthes candida Herb (Amaryllidaceae)
《Journal of Integrative Plant Biology》2006年第2期223-231,共9页Chuan-Fang Wu Jian Li Jie An Li-Qing Chang Fang Chen Jin-Ku Bao 
Supported by the National Natural Science Foundation of China (30000032).
A novel mannose-bindlng aggiutinln was purified from bulbs of Zephyranthes candida Herb by extraction, precipitation with 80% (NH4)2SO4, and ion-exchange chromatography on DEAE-Sepharose followed by gel flitration o...
关键词:agglutinating activity insecticidal activity molecular clone sequence analysis Zephyranthes candida agglutinin. 
Anti-HIV Ⅰ/Ⅱ Activity and Molecular Cloning of a Novel Mannose/Sialic Acidbinding Lectin from Rhizome of Polygonatum cyrtonema Hua被引量:9
《Acta Biochimica et Biophysica Sinica》2006年第2期70-78,共9页Jie AN Jin-Zhi LIU Chuan-Fang WU Jian LI Lei DAI Els Van DAMME Jan BALZARINI Erik De CLERCQ Fang CHEN Jin-Ku BAO 
This work was supported by a grant from the National Natural Science Foundation of China (No. 30000032)
The anti-human immunodeficiency virus (HIV) Ⅰ/Ⅱ activity of a mannose and sialic acid binding lectin isolated from rhizomes of Polygonatum cyrtonema Hua was elucidated by comparing its HIV infection inhibitory act...
关键词:Polygonatum cyrtonema HUA mannose-binding lectin anti-human immunodeficiency virus(HIV) Ⅰ/Ⅱ molecular cloning 3'/5'-rapid amplification of cDNA ends (RACE) sequence alignment molecular modeling docking 
红花石蒜球茎凝集素的纯化及部分性质被引量:17
《应用与环境生物学报》2005年第2期164-167,共4页常丽青 吴传芳 吕鸿周 刘超 顾莹 陈放 吴洽庆 鲍锦库 
国家自然科学基金资助项目 (No. 30000032 )~~
石蒜科植物红花石蒜(Lycoris radiata)球茎经生理盐水浸取、硫酸铵分级沉淀、DEAE-Sepharose和SephacrylS-100分子筛层析得到石蒜凝集素(L.radiata agglutinin,LRA).经SDS-PAGE检测为单一蛋白带,亚基相对分子量Mr为12×103,Sephacryl S-...
关键词:石蒜凝集素(LRA) 纯化 凝集活性 促淋巴细胞有丝分裂活性 抗病毒活性 
风雨花甘露糖结合凝集素基因克隆与序列分析被引量:2
《生物医学工程学杂志》2004年第5期812-818,共7页鲍锦库 吴传芳 安洁 高顺 赵曦 常丽青 荣艳珍 王陈继 陈放 
国家自然科学基金资助项目 ( 3 0 0 0 0 0 3 2 )
单子叶甘露糖结合凝集素具有抑制人类免疫缺陷病毒的活性。风雨花 (Zephyranthes grandiflora)球茎中含有亚基分子量为 12 KD的甘露糖结合凝集素 (ZGA)。运用同源克隆的方法设计简并引物 ,通过 3'和 5 'RACE技术 ,从风雨花总 RNA中克隆...
关键词:甘露糖结合凝集素 KD 蛋白序列 人类免疫缺陷病毒 基因克隆 前体蛋白 序列分析 简并引物 结构域 氨基酸序列 
Effects of Denaturation and Amino Acid Modification on Fluorescence Spectrum and Hemagglutinating Activity of Hericium erinaceum Lectin被引量:7
《Acta Biochimica et Biophysica Sinica》2004年第5期343-350,共8页MengGONG JieAN Hong-ZhouLü Chuan-FangWU Yi-JinLI Jing-QiuCHENG Jin-KuBAO 
This work was supported by a grant from the National Natural Science Foundation of China (General Program) (No. 30000032)
A sialic acid-binding lectin (Hericium erinaceum lectin, HEL), isolated from fresh fruiting bodies of Hericium erinaceum, was treated with various temperature and pH to investigate its fluorescence spectra a...
关键词:sialic acid-binding lectin Hericium erinaceum lectin fluorescence spectrum and fluores-      cence quenching hemagglutinating activity chemical modification 
检索报告 对象比较 聚类工具 使用帮助 返回顶部