supported by Ministry of Science and Technology (National Key Research and Development Program of China,No.2018YFA0901900);the National Natural Science Foundation of China (Nos.21931014,21525209, 21621002,21772225,and 21761142003);Chinese Academy of Sciences (Strategic Priority Research Program,No.XDB20000000;International Partner Program,No.121731KYSB20190039;Key Research Program of Frontier Sciences,No.QYZDB-SSW-SLH040);Science and Technology Commission of Shanghai Municipality (No.17XD1404600);K.C.Wong Education Foundation。
Chemoselective amine bioco njugation has long been a challenge for native protein modification.Inspired by Thiele’s seminal discovery,Li and co-workers recently developed an orto-phthalaldehyde(OPA)based reagent for ...