This study was supported by the National Key Research and Development Program of China(grant nos.2021YFC2100600,2019YFA0905100 and 2018YFA0901600);the National Natural Science Foundation of China(grant nos.31872614,32022002,21977113);the Youth Scientists Innovation Promotion Association of CAS(2019090)to S.S.G.,Innovative Cross Team project of Chinese Academy of Sciences,CAS(grant no.JCTD-2019-06);Shandong Provincial Natural Science Foundation(Major Basic Research Projects)(grant no.ZR2019ZD18).
Microbial cell factories(MCFs)and cell-free systems(CFSs)are generally considered as two unrelated approaches for the biosynthesis of biomolecules.In the current study,two systems were combined together for the overpr...