This work was supported by grants from National Ba-sic Research Program of China(973 Program)(No:001CB509903,001CB509904);Hi-Tech Research and Development Program of China(973 Program)(No:2001A A216121.2004AA205010);National Natural Science Foun-dation of China(No:30040003);Science and Technology Committee of Shanghai Municipality(No:99DJ 14002,00DJI 4033,0IDJ 14003.03DJ 14017);Chinese Academy of Science(No:KSCX-2-3-08);Shanghai Municipal Edu-cation Commission and Shanghai Second Medical University.
Six human embryonic stem cell lines were established from surplus blastocysts. The cell lines expressed alkaline phosphatase and molecules typical of primate embryonic stem cells, including Oct-4, Nanog, TDGF1, Sox2, ...