the National Key Research and Development Program of China(2017YFA0105103,2021YFA0805903);the National Natural Science Foundation of China(81941004,32170542);2020 Research Program of Sanya Yazhou Bay Science and Technology City(202002011);Major Science and Technology Projects of Hainan Province(ZDKJ2021030);Key Research&Development Program of Hainan Province(ZDYF2021SHFZ052);Youth Innovation Promotion Association of the Chinese Academy of Sciences(2019347);Young Elite Scientist Sponsorship Program by CAST(YESS20200024);Biological Resources Progaramme,Chinese Academy of Sciences(KFJBRP-017-57);Key Research&Development Program of Bioland Laboratory(Guangzhou Regenerative Medicine and Health Guangdong Laboratory)(2018GZR110104004);China Postdoctoral Science Foundation(2020M682943);Science and Technology Planning Project of Guangdong Province,China(2019A030317010,2020B1212060052,2021B1212040016,2021A1515011110);Science and Technology Program of Guangzhou,China(202007030003);Research Unit of Generation of Large Animal Disease Models,Chinese Academy of Medical Sciences(2019-I2M-5-025)。
Inducible expression systems are indispensable for precise regulation and in-depth analysis of biological process.Binary Tet-On system has been widely employed to regulate transgenic expression by doxycycline.Previous...