KNOCK-IN

作品数：26被引量：105H指数：5
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相关领域：生物学医药卫生更多>>
相关作者：王红胡静姜元于翠娟吴智群更多>>
相关机构：吉林大学陕西师范大学广西大学国立中兴大学更多>>
相关期刊：《Journal of Integrative Agriculture》《Translational Neurodegeneration》《Genomics, Proteomics & Bioinformatics》《Neuroscience Bulletin》更多>>
相关基金：国家自然科学基金中国博士后科学基金广东省自然科学基金北京市自然科学基金更多>>
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Knock-in of exogenous sequences based on CRISPR/Cas9 targeting autosomal genes and sex chromosomes in the diamondback moth,Plutella xylostella
《Journal of Integrative Agriculture》2024年第9期3089-3103,共15页Shanyu Li Guifang Lin Haoqi Wen Haiyan Lu Anyuan Yin Chanqin Zheng Feifei Li Qingxuan Qiao Lu Jiao Ling Lin Yi Yan Xiujuan Xiang Huang Liao Huiting Feng Yussuf Mohamed Salum Minsheng You Wei Chen Weiyi He 
supported by the National Natural Science Foundation of China(32172503 and 32260721);the Natural Science Foundation of Fujian Province,China(2023J01069);the State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops,China(SKL2022001);the Innovation Fund of Fujan Agriculture and Forestry University,China(KFB23014A);the Undergraduate Training Program for Innovation and Entrepreneurship of Fujian Province,China(S202210389101).
Genetic pest control strategies based on precise sex separation and only releasing sterile males can be accomplished by site-specific genome editing.In the current study,we showed that the mutation of single-allele Px...
关键词：cruciferous specialist fl(2)d CRISPR/Cas9 KNOCK-IN sex chromosome 
The collagen type Ⅰ alpha 1 chain gene is an alternative safe harbor locus in the porcine genome被引量：1
《Journal of Integrative Agriculture》2023年第1期202-213,共12页XIANG Guang-ming ZHANG Xiu-ling XU Chang-jiang FAN Zi-yao XU Kui WANG Nan WANG Yue CHE Jing-jing XU Song-song MU Yu-lian LI Kui LIU Zhi-guo 
supported by the Major Scientific Research Tasks for Scientific and Technological Innovation Projects of the Chinese Academy of Agricultural Sciences(CAAS-ZDRW202006);the National Transgenic Breeding Project(2018ZX08010-10B);the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences(ASTIP-IAS05).
Efficient and stable expression of foreign genes in cells and transgenic animals is important for gain-of-function studies and the establishment of bioreactors.Safe harbor loci in the animal genome enable consistent o...
关键词：COL1A1 gene safe harbor KNOCK-IN CRISPR/Cas9 PIG 
Comparing successful gene knock-in efficiencies of CRISPR/Cas9 with ZFNs and TALENs gene editing systems in bovine and dairy goat fetal fibroblasts被引量：11
《Journal of Integrative Agriculture》2018年第2期406-414,共9页LIU Hui LIU Chang ZHAO Yu-hang HAN Xue-jie ZHOU Zheng-wei WANG Chen LI Rong-feng LI Xue-ling 
supported by the National Transgenic Project of China (2016ZX08010001-002);the National Natural Science Foundation of China (81471001);the Inner Mongolia Science and Technology Program, China (201502073);the National 863 Prgram of China (2009AA10Z111)
This study aimed to compare the efficiencies of clustered regulatory interspaced short palindromic repeat（CRISPR）/Cas9-mediated gene knock-ins with zinc finger nucleases（ZFNs） and transcription activator-like effe...
关键词：myostatin（MSTN） β-casein（CSN2） bovine fetal fibroblasts CRISPR/Cas9 dairy goat fetal fibroblasts eGFP hFat-1 knock-in mutation efficiency TALENs ZFNs 
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