supported by the National Key Research and Development Program of China(2020YFA0907800);the National Natural Science Foundation of China(32170095 and 32100066);the Youth Innovation Promotion Association CAS(Y202027)to W.W.
Parageobacillus thermoglucosidasius is emerging as a highly promising thermophilic organism for metabolic engineering.The utilization of CRISPR-Cas technologies has facilitated programmable genetic manipulation in P.t...
financially supported by the National Natural Science Foundation of China (21922601, 51808096);Liaoning Revitalization Talents Program (XLYC1807080);the Fundamental Research Funds for the Central Universities (DUT20YG123)
We found U2R1,a previously discovered G-rich DNA sequence,could form the G-quadruplex(G4)structure at high tem-perature and maintain high stability.By utilizing its specific binding ability with the N-methylmesoporphy...
The research was supported financially by the University of Technology Sydney.All procedures were approved by the UTS Animal Care and Ethics Committee(protocol ACEC 2012000256);a NSW National Parks and Wildlife Service scientific licence(SL 101013 toJ.K.W.).
Fluctuations in food availability occur in many ecosystems and pose challenges to the organisms that inhabit them(Berg et al.2017).Because lizards have significantly lower field metabolic rates than endotherms,they ar...
supported by INNOVA-CORFO 07CN13PXT-64;Instituto Antártico Chileno (INACH);US Air Force Office of Scientific Research (AFOSR)
Glutamate dehydrogenase (GDH) catalyzes the oxidative deamination of glutamate to a-ketoglutarate and ammonium ions. Currently the determination of ammonium and glutamate is carried out using a bovine GDH enzyme, wh...
Biocatalysts are intrinsically reactive and hence their operational stability is of vital significance for any bioprocess. The setback in biocatalyst stability has been tackled from diverse prospects. Inherently, stab...
Protein domain swapping is an efficient way in protein functional evolution in vivo and also has been proved to be an effective strategy to modify the function of the multi- domain proteins in vitro. To explore the po...
The discovery of new, highly active, biomass-degrading enzymes is important to the development of a sustainable biofuels industry. Dictyoglomus turgidum, a thermophilic, anaerobic eubacterium that ferments cellulose a...
The occurrence frequencies of the dinucleotides of genes of three thermophilic and three mesophilic species from both archaea and eubacteria were investigated in this study. The genes encoding water soluble proteins w...
This work was supported by grants from the National Natural Science Foundation of Chain (No. 30870518), the National High Technology Research and Development Program of China (No. 2007AA100601-2) and the Platform base construction projects of Jinlin University.
A gene encoding a special thermophilic multifunctional amylase OPMA-N was cloned from Bacillus sp. ZW2531- 1. OPMA-N has an additional 124-residue N-terminal domain compared with typical amylases and forms a relativel...
Project(200805032) supported by Grands from Scientific Research Program of Marine Public Welfare Industry of China;Project (DYXM-115-02-2-07) supported by China Ocean Mineral Resources R&D Association (COMRA)
Culturable thermophilic microorganisms were enriched from samples collected from Lau Basin hydrothermal vents in artificial seawater medium at 45 ℃ and pH 7.0. Microbial diversities of the enriched communities were d...