This work was funded by National Natural Science Foundation of China(32272279);the Key R&D project of Qingdao Science and Technology Plan(22-3-3-hygg-29-hy).
Alpha-lactalbumin(α-LA)is a major whey protein found in breast milk and plays a crucial role in the growth and development of infants.In this study,Bacillus subtilis RIK1285 harboring AprE signal peptide(SP)was selec...