National Basic Research Program of China (973 program, 2013CB932800);the National Natural Science Foundation of China (NSFC, 31100524 to M.Z., 31170817 for C.T., and 20972148 to L.L.)
The C-terminal conjugate of ubiquitin with 7-amino-4-methylcoumarin (Ub-AMC) is an important probe for fluorescencebased analysis of deubiquitinating enzyme (DUB) activity. It is important to develop more efficien...