Project supported by the National Natural Science Foundation of China (No. 21075097).Acknowledgements We would like to thank Ms. Cui-Zhu Lu for her scientific support and helpful discussion.
The unfolding of bovine heart cytochrome c induced by urea and guanidine hydrochloride was studied through their intrinsic fluorescence emission spectra, fluorescence phase diagrams, fluorescence quenching, size-exclu...