This work is supported by the National Basic Research Program of China(973 Program)under grant No.2013CB932804;the National Natural Science Foundation of China under Grant No.11574329 and 11322543;the Science and Technology Planning Project of General Administration of Quality Supervision,Inspection and Quarantine(AQSIQ)of PR China under Grant No.2015IK011;AQSIQ industrial public service scientific research project of the Ministry of Science and Technology of P.R.China under Grant No.201410049;The YS101 type high sensitive detector of chemiluminescence was manufactured by Yishang Innovation Technology Co.,Ltd.
FoF1-ATPase is an active rotary motor,and generates three-ATP for each rotation.At saturated substrate concentration,the motor can achieve about 103 r.p.m,which means one motor can generate about 105 ATP molecules dur...