This work was funded by the Ministry of Science and Technology of China(Grant Nos.2006AA02A316,2009DFB30310 and 2006CB910901);the National Natural Science Foundation of China(Grants Nos.30670427 and 30721003);the Ministry of Health of China(Grant No.2008ZX10404);CAS Research Grant(No.KSCX2-YW-R-127 and INFO-115-D01-2009).
Sometimes crystals cannot diffract X-rays beyond 3.0Åresolution due to the intrinsic flexibility associated with the protein.Low resolution diffraction data not only pose a challenge to structure determination,but als...