视网膜细胞

作品数:325被引量:554H指数:10
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视黄酸联合视网膜细胞培养上清液对胚胎干细胞体外分化的诱导作用被引量:2
《眼科学报》2003年第2期122-125,共4页张良 唐仕波 罗燕 黄冰 张淳 陈系古 
国家自然科学基金(30070803);国家杰出青年基金(30125041)
目的:探讨经过初步诱导的拟胚体在视黄酸和视网膜细胞培养上清液作用下的分化特征。方法:将胚胎干细胞(embryonic stem cells,ESC)自液氮中复苏、培养,传1代后进行拟胚体(embry-onic bodies,EB)培养。3天半的 EB离心重悬后不作消化,使...
关键词:视黄酸 视网膜细胞 上清液 胚胎干细胞 细胞分化 实验 
Cloning and Sequence Analysis of Light Variable Region Gene of Anti-human Retinoblastoma Monoclonal Antibody
《眼科学报》2002年第3期185-189,共5页Xiufeng Zhong, Yongping Li, Shuqi Huang, Bo Ning Chunyan Zhang, Jianliang Zheng, Guanguang FengZhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China 
Supported in part by Grants 39870801,39400144 from National Natural Sciences Foundation of P.R.China;by Grants 98011 from Natural Sciences Foundation of Guangdong Province and by Grants 98007 from 211 Project Foundation of Sun Yat-Sen University of Medic
Purpose: To clone the variable region gene of light chain of monoclonal antibody against human retinoblastoma and to analyze the characterization of its nucleotide sequence as well as amino acid sequence.Methods: Tota...
关键词:抗人成视网膜细胞瘤 单克隆抗体 轻锭 可变区基因 基因克隆 序列分析 
Exon-by-exon Screening for RB Germline Mutations Using Heteroduplex-SSCP Analysis被引量:1
《眼科学报》1997年第1期5-11,共7页Qingjiong Zhang, Kensei Minoda, Ruiping Zeng, Zhongyao Wu Xueshan Xiao, Shiqiang Li, Fengshen ZhangZhongshan Ophthalmic Center Department of Medica GeneticsSun Yat-sen University of Medical Sciences , Guangzhou 510060 , ChinaDepartment of Ophthalmology, Teikyo University Ichihara Hospital, Ichihara , Chiba 299 - 01, Japan. 
Guang Dong Provincial Natural Science Foundation (QZ:930354, 960149 );the Misnistry of Public Health(QZ);the Ministry of Health and Welfare of Japan(KM);the Mishima Fund for Eye Research and International Exchange(QZ);the Sasagawa Medical Re
Objective: To disclose the nature of RBI germline mutations in Chinese and to develop a practical and effective way for mutational screening. Methods: Leukocyte DNA was prepared from 8 Chinese patients with hereditary...
关键词:成视网膜细胞瘤 突变 SSCP 遗传因素 非遗传因素 
Electrophoretic Determination of Aqueous and Serum Neuron-specific Enolase in the Diagnosis of Retinoblastoma
《眼科学报》1997年第1期12-16,共5页Zhongyao Wu, Huasheng Yang, Suhua Pan, Zhicong ChenZhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences , Guangzhou 510060 , China 
Purpose: Neuron-specific enolase (NSE) of containing γ-enolase is considered valuable in the diagnosis of tumours of neuroectodermal origin.Method : We used rapid electrophoretic method on cellulose acetate plate to ...
关键词: 免疫电泳 水状体 血清 成视网膜细胞瘤 神经元性烯醇酶 
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