MUTAGENESIS

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An in vitro recombination-based reverse genetic system for rapid mutagenesis of structural genes of the Japanese encephalitis virus
《Virologica Sinica》2015年第5期354-362,共9页Ruikun Du Manli Wang Zhihong Hu Hualin Wang Fei Deng 
supported by grants from the National Science Foundation of China (NSFC) (No.31125003 and No.31321001);the National Basic Research Program (973 Program) of China (Grants 2010CB530103)
Japanese encephalitis virus(JEV) is one of the most common pathogens of severe viral encephalitis, which is a severe threat to human health. Despite instability of the JEV genome in bacteria, many strategies have been...
关键词：JAPANESE ENCEPHALITIS virus(JEV) REVERSE genetic s 
A novel transposable Mu-like prophage in Bacillus alcalophilus CGMCC 1.3604(ATCC 27647)被引量：1
《Virologica Sinica》2015年第1期63-65,共3页Junjie Yang Yimeng Kong Xuan Li Sheng Yang 
supported by the National Basic Research Program(973 Program)of China(2014CB745100);the National Key Technologies R&D Program of China(2012AA022101)
Dear Editor,Transposable phages,which are reproduced by transposition(Harshey,2012;Taylor,1963),have been widely applied in the field of biotechnology to manipulate operon/gene fusions,in vivo cloning,randomion mutage...
关键词：cloning biotechnology reproduced MUTAGENESIS PHAGE sequencing DNA http Sanger CHROMOSOME 
Mutagenesis of Ser^(24) of cytochrome b559 α subunit affects PSII activities in Chlamydomonas reinhardtii
《Chinese Science Bulletin》2007年第7期896-902,共7页MA JingJing LI LiangBi JING YuXiang KUANG TingYun 
Supported by the National Basic Research Program (973 Program) (Grant No. G1988010100);the National Natural Science Foundation of China (Grant No. 088121A)
In order to study the functions of cytochrome b559 (Cyt b559) in photosystem two (PSII) activity, mutant S24F of Chlamydomonas reinhardtii was constructed using site directed mutagenesis, in which Serine24 (Ser24) loc...
关键词：莱茵衣藻 细胞色素b559α亚基 丝氨酸残基 Ser24 突变 光系统II 光合活性 
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