The present study aimed to completely remove estrogens, including oestrone(E1), oestradiol(E2), oes-triol(E3), 17a-ethinylestradiol(EE2) and bisphenol-A(BPA), from soil using Pseudomonas putida(P., putida)...
Supported by the Natural Science Foundation of Shanxi Province(No.20031042).
A 1440bp open-reading frame encoding D-hydantoinase from Pseudomonas putida YZ-26 was cloned and sequenced( GenBank AY387829). The DNA fragment was inserted into Nde and BamHI sites of vector pET-3a, yielding a reco...