SEQUENCING

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Accurate quantification of 3'-terminal 2'-O-methylated small RNAs by utilizing oxidative deep sequencing and stem-loop RT-qPCR被引量：2
《Frontiers of Medicine》2022年第2期240-250,共11页Yan Kong Huanhuan Hu Yangyang Shan Zhen Zhou Ke Zen Yulu Sun Rong Yang Zheng Fu Xi Chen 
This work was supported by the Fundamental Research Funds for the Central Universities(No.020814380146);National Basic Research Program of China(973 Program)(No.2014CB542300);National Natural Science Foundation of China(Nos.32022015,32001077,31871295,21877060,81250044,81602697,and 81772727);Research Unit of Extracellular Non-Coding RNA,Chinese Academy of Medical Sciences(No.2021RU015).
The continuing discoveries of novel classes of RNA modifications in various organisms have raised the need for improving sensitive,convenient,and reliable methods for quantifying RNA modifications.In particular,a subs...
关键词：small RNAs 2'-0-methylation SEQUENCING RT-QPCR 
Genetic Analysis of the PKHD1 Gene with Long-rang PCR Sequencing
《Journal of Huazhong University of Science and Technology(Medical Sciences)》2016年第5期758-766,共9页童永清 刘蓓 付朝泓 郑红云 顾剑 刘航 罗洪波 李艳 
supported by grants from the Hubei Provincial Natural Science Foundation of China(No.2010CDB-06903);National Key Basic Research Program of China(“973”Program,No.2012CB526706);the National Natural Science Foundation of China(Nos.81000771 and 81271694)
PKHD1 gene mutations are found responsible for autosomal recessive polycystic kidney disease（ARPKD）. However, it is inconvenient to detect the mutations by common polymerase chain reaction（PCR） because the open re...
关键词：sequencing Sequencing recessive screening detecting conclusion applicable mutation genomic digestion 
Bacterial diversity and community structure in the East China Sea by 454 sequencing of the 16S rRNA gene被引量：8
《Chinese Journal of Oceanology and Limnology》2014年第3期527-541,共15页董逸 赵苑 张文燕 李艳 周锋 刘诚刚 吴莹 刘素美 张武昌 肖天 
Supported by the National Basic Research Program of China(973 Program)(No.2011CB409804);the National Natural Science Foundation of China(No.41121064)
The 454 sequencing method was used to detect bacterial diversity and community structure in the East China Sea. Overall, 149 067 optimized reads with an average length of 454 nucleotides were obtained from 17 seawater...
关键词：bacterial diversity community structure 454 sequencing water masses canonical correspondence analysis East China Sea 
Sequencing and expression analysis of CD3γ/δ and CD3ε chains in mandarin fish, Siniperca chuatsi被引量：2
《Chinese Journal of Oceanology and Limnology》2013年第1期106-117,共12页郭政 聂品 
Supported by the National Natural Science Foundation of China (No.U0631010),the Government of Guangdong Province;the National Basic Research Program of China (973 Program) (No. 2009CB118703)
The genomic and cDNA sequences of the CD3γ/δ and CD3ε homologues in the mandarin fish, Siniperca chuatsi, were determined. As in other vertebrate CD3 molecules, the deduced amino acid sequences of mandarin fish CD3...
关键词：CD3γ/δ CD3ε mandarin fish MITOGEN Flavobacterium columnare 
BAC end sequencing of Pacific white shrimp Litopenaeus vannamei: a glimpse into the genome of Penaeid shrimp被引量：1
《Chinese Journal of Oceanology and Limnology》2012年第3期456-470,共15页赵翠 张晓军 柳承璋 郇聘 李富花 相建海 黄超 
Supported by the National Natural Science Foundation of China (Nos.30972245, 30730071);the National Basic Research Program of China (973 Program) (No. 2012CB114403)
Little is known about the genome of Pacific white shrimp (Litopenaeus vannamei). To address this, we conducted BAC (bacterial artificial chromosome) end sequencing of L. vannamei. We selected and sequenced 7 812 BAC c...
关键词：Litopenaeus vannamei BES repetitive sequences SSR genome survey 
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