Supported by the National Key Research and Development Plan of China(2019YFC1905902,2019YFC1905900);Key Research and Development Plan in Shandong Province(2020CXGC010603,2021ZDSYS10,2022CXGC020206);"Open Competition Mechanism"Project of Qilu University of Technology(Shandong Academy of Sciences)(2022JBZ01-06);Taishan Industry Leading Talent Program(tscy20180103);National Natural Science Foundation of China(31801527)。
Each possible pair of residues inβ-1,4 glucanase for disulfide formation was assessed using online websites,and four pairs L28C-S256C,Q41C-P278C,S122C-N163C and A184C-A215C were selected.Accordingly,four recombinant ...