partially supported by the Program 30470120,30671419,30700541 from the National Natural Science Foundation of China;by the 863 Programs 2006AA10Z108,2006AA100108 from the Ministry of Science and Technology of China;by the Ph.D Funding 20050307009 from the Ministry of Education of China;by the Program BK2006139 from the Natural Science Foundation of Jiangsu Province;by Research Fund KJ05006 from Nanjing Agricultural University,China.
A simple and efficient method for cloning the flanking genomic sequences of a known DNA region is reported in this study. This method combined partial restriction endonuclease digestion, adaptor ligation, and a single...