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Single-cell transcriptomes and runx2b^(-/-)mutants reveal the genetic signatures of intermuscular bone formation in zebrafish被引量:19
《National Science Review》2022年第11期92-102,共11页Chun-Hong Nie Shi-Ming Wan Yu-Long Chen Ann Huysseune Ya-Ming Wu Jia-Jia Zhou Alexandre Wagner Silva Hilsdorf Wei-Min Wang Paul Eckhard Witten Qiang Lin Ze-Xia Gao 
supported by the earmarked fund for China Agriculture Research System (CARS-45-01);Hubei Hongshan Laboratory (2021hszd001);National Natural Science Foundation of China (31872559, 41825013 and U21A20263);National Key Research and Development Program of China(2018YFD0900102);Laboratory for Lingnan Modern Agriculture (NZ2021011);Special Project of Science and Technology Innovation of the Ministry of Education (2021ZL08);Wuhan Applied Foundational Frontier Project (2020020601012253);Key Research Program of Frontier Sciences of CAS (ZDBSLY-DQC004)
Intermuscular bones(IBs)are mineralized spicules,present in the myosepta of many,but not all,teleost species.IBs are often small and sharp,and they consequently limit how the fish can be processed;the IBs may cause in...
关键词:TELEOSTS ZEBRAFISH intermuscular bones scRNA-seq tendon-osteoblast cell lineage runx2b genetic breeding 
翘嘴鲌Runx2b基因的克隆与表达特征分析
《浙江农业学报》2024年第5期1024-1031,共8页何昌熙 郑建波 马建波 贾永义 刘士力 蒋文枰 迟美丽 程顺 李飞 
浙江省农业(水产)新品种选育重大科技专项(2021C02069-3);浙江省自然科学基金(LTGN23C190008)。
为探究Runx2b基因在翘嘴鲌肌间刺骨化过程中的调控作用,基于转录组数据和PCR扩增技术克隆获得了翘嘴鲌Runx2b基因全长编码序列。通过茜素红整体骨骼染色对肌间刺(IBs)形成的不同阶段进行了分期,同时采用qRT-PCR技术检测了Runx2b基因的...
关键词:翘嘴鲌 肌间刺 Runx2b 基因表达 骨化 
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