口腔鳞状细胞癌中p53、EGFR表达与临床病理及预后的相关性研究  

Correlation between expression of P53 and EGFR in oral squamous cell carcinoma and clinicopathology and prognosis

作　　者：李至睿 李晓粤 刘爱军[2] 储冰峰[1] 林燕蝶 LI Zhi-rui;LI Xiao-yue;LIU Ai-jun;CHU Bing-feng;LIN Yan-die(Department of Stomatology,The first Medical Center of Chinese PLA General Hospital,Beijing 100039,China;Department of Pathology,The seventh Medical Center of Chinese PLA General Hospital,Beijing 100070,China)

机构地区：[1]解放军总医院第一医学中心口腔科,北京100853 [2]解放军总医院第七医学中心病理科,北京100700

出　　处：《诊断病理学杂志》2023年第10期987-991,1057,共6页Chinese Journal of Diagnostic Pathology

摘　　要：目的本研究旨在评估p53、EGFR在口腔鳞状细胞癌(OSCC)中的表达情况,探讨其与组织分化、T、N、临床分期及预后之间的关系。方法回顾102例经手术治疗的OSCC患者,经免疫组化检测肿瘤组织中EGFR、p53蛋白的表达水平,结合患者临床病理资料,使用SPSS 21.0对数据进行统计学分析。结果在OSCC患者中,肿瘤分化程度高低与临床分期及淋巴结转移情况具有相关性(Pearson R=0.38,P<0.001;Pearson R=0.25,P=0.008);p53在OSCC中的阳性表达率为63.7%,p53(突变型)表达与肿瘤分化程度具有相关性(Pearson R=0.3,P=0.002);EGFR在OSCC中的阳性表达率为70.6%,伴随肿瘤大小及浸润程度的进展,EGFR表达增加,且差异具有统计学意义(P=0.02)。Kaplan-Meier法分析结果显示:p53(突变型)组OSCC患者3年无进展生存期(PFS)显著低于p53(null型)和p53(野生型)组,且差异具有统计学意义(P=0.04),但EGFR(-)与EGFR(+)OSCC患者3年无进展生存期(PFS)无显著差别。结论p53和EGFR蛋白在口腔鳞癌组织中高表达,p53(突变型)与肿瘤分化程度相关,并提示较差预后,EGFR表达与肿瘤T分期相关,提示p53和EGFR蛋白可能是OSCC发生、发展的潜在重要因素。Objective This study aimed to evaluate the expression of p53 and EGFR in oral squamous cell carcinoma(OSCC)and explore their relationship with tissue differentiation,T and N stage,clinical stage,and prognosis.Methods A retrospective analysis was conducted on 102 OSCC patients who underwent surgical treatment.Immunohistochemical staining was performed to determine the expression levels of EGFR and p53 in tumor tissues.Clinical and pathological data were collected and analyzed using SPSS 21.0.Results In OSCC patients,the degree of tumor differentiation was correlated with clinical stage and lymph node metastasis(Pearson R=0.38,P<0.001;Pearson R=0.25,P=0.008).The positive expression rate of p53 in OSCC was 63.7%,and p53(mutation)expression was correlated with tumor differentiation(Pearson R=0.3,P=0.002).The positive expression rate of EGFR in OSCC was 70.6%,and its expression increased with tumor size and infiltration,with significant differences(P=0.02).Kaplan-Meier analysis showed that OSCC patients with p53(mutation)had a significantly lower 3-year progression-free survival(PFS)compared to those with p53(null)and p53(wild type),with statistical significance(P=0.04).However,there was no significant difference in 3-year PFS between EGFR(-)and EGFR(+)OSCC patients.Conclusion p53 and EGFR proteins were highly expressed in oral squamous cell carcinoma tissues.p53(mutation)was associated with tumor differentiation and indicated a poorer prognosis.EGFR expression was correlated with tumor T stage.These findings suggest that p53 and EGFR may be potential important factors in the occurrence and development of OSCC.

关 键 词：P53 EGFR 口腔鳞状细胞癌 临床病理 预后 

分 类 号：R780.2[医药卫生—口腔医学]