重组运动发酵单胞菌乙醇发酵效果的研究  

Study on Ethanol Fermentation Effect of Recombinant Zymomonas Mobilis

作　　者：李北 汪浩勇[2] William Wang 李永恒 郭春雷 黄加军 LI Bei;WANG Haoyong;William Wang;LI Yongheng;GUO Chunlei;HUANG Jia-jun(Guangxi COFCO Bio-energy Co.,Ltd.,Beihai Guangxi 536100,China;Key Laboratory of Fermentation Engineering of Ministry of Education,Hubei University of Technology,Wuhan Hubei 410068,China;Harriton High School,Rosemont PA USA 19010)

机构地区：[1]广西中粮生物质能源有限公司,广西北海536100 [2]湖北工业大学发酵工程教育部重点实验室,湖北武汉430068 [3]Harriton High School,Rosemont,PA USA 19010

出　　处：《当代化工》2020年第7期1400-1405,共6页Contemporary Chemical Industry

基　　金：广西重点研发计划,多元原料高效生产燃料乙醇关键技术与产业化应用示范(项目编号:桂科AB192245049)

摘　　要：运动发酵单胞菌通过ED途径高效转化单糖生成乙醇,其生长不需氧气,能够以N2作为氮源,抗逆性强;其乙醇发酵速度快,乙醇理论产率高,生产周期较短和原料利用率较高,容易迚行基因工程改造。本文采用水稻和木薯为乙醇发酵的原料,研究重组运动发酵单胞菌的发酵特点,验证其乙醇发酵性能,为其今后在乙醇生产中实际使用积累经验和提供参考依据。本实验结果证明,与安琪超级酿酒干酵母相比,在重组运动发酵单胞菌的乙醇发酵过程中,CO2产量更低导致发酵失重更少,乙醇产量明显增加,最佳发酵温度更高,淀粉出酒率更高和淀粉利用率更高。重组运动发酵单胞菌对安菌泰和青霉素有较强的耐受性,乙醇发酵指数期大约为0~40 h,种子扩大培养期间不需要添加任何外来氮源。Zymomonas mobilis can efficiently transform monosaccharides to produce ethanol through ED pathway.Its growth does not require oxygen,can use N2 as a nitrogen source and is highly resistant to stress.Its ethanol fermentation speed is fast and the production cycle is short.Its theoretical ethanol yield is high and the raw material utilization rate is high.It is easy to carry out gene manipulation for genetic transformation.In this paper,ethanol fermentation of new typed recombinant Z.mobilis strains was studied by using rice or cassava as raw materials.Compared with the Angel yeast of Saccharomyces cerevisiae,in the ethanol fermentation process,the CO2 production of the Z.mobilis is significantly lower,and the ethanol production is significantly higher.Its optimal fermentation temperature is higher,starch liquor yield and starch utilization rate are significantly higher,and acid production is lower.It has a strong tolerance to Penicillin.The ethanol fermentation index period is between 0~40 h,and no external nitrogen source is needed during its seed expansion cultivation.

关 键 词：运动发酵单胞菌 乙醇发酵 水稻 木薯 

分 类 号：TQ223.122[化学工程—有机化工] TQ920.6[轻工技术与工程—发酵工程]