Reconstitution of membrane contact by unilamellar vesicles  

作　　者：Shulin Li Min Zhang Liang Ge 

机构地区：[1]State Key Laboratory of Membrane Biology,Beijing 100101,China [2]Tsinghua-Peking Center for Life Sciences,Beijing 100084,China [3]School of Life Sciences,Tsinghua University,Beijing 100084,China [4]School of Pharmaceutical Sciences,Tsinghua University,Beijing 100084,China

出　　处：《Biophysics Reports》2023年第4期188-194,共7页生物物理学报（英文版）

基　　金：funded by National Natural Science Foundation of China(32225013,32130023,9225430003,31872832);Ministry of Science and Technology of the People’s Republic of China(2021YFA0804802,2019YFA0508602);Beijing Natural Science Foundation(JQ20028);supported by Vanke Special Fund for Public Health and Health Discipline Development,Tsinghua University(2022Z82WKJ009)

摘　　要：Eukaryotic cells compartmentalize diverse biochemical functions within organelles defined by intracellular membranes.Recent focus has intensified on studying the interactions among organelles and the role of membrane contacts in maintaining cellular balance.While analyzing these contacts mainly involves fluorescence and electron microscopy,as well as biochemical cell fractionation,understanding their mechanisms and responses to genetic and environmental changes remains challenging.Here we describe an approach employing in vitro reconstitution of membrane contacts using unilamellar vesicles.This technique offers insights into contact mechanisms when combined with established methods like fluorescence imaging and mass spectrometry,potentially deepening our understanding of membrane contacts and organelle networks.

关 键 词：Membrane contacts Organelle interaction network Giant unilamellar vesicle(GUV) Liposome ER-Golgi intermediate compartment(ERGIC) ER-exit sites(ERES) COPII TMED9 SEC12 Autophagy AUTOPHAGOSOME 

分 类 号：Q244[生物学—细胞生物学]