猕猴桃多酚的纯化工艺及组分分析  

Purification process and component analysis of kiwifruit polyphenols

作　　者：邵佩 陈胜 罗威 苗丽坤 冯武[2] SHAO Pei;CHEN Sheng;LUO Wei;MIAO Likun;FENG Wu(Wuhan Huanghelou Flavors and Fragrances Co.,Ltd.,Wuhan 430040;College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070)

机构地区：[1]武汉黄鹤楼香精香料有限公司,武汉430040 [2]华中农业大学食品科学技术学院,武汉430070

出　　处：《食品科技》2019年第12期260-265,共6页Food Science and Technology

基　　金：中央高校基本科研业务费专项基金项目(2662016PY100).

摘　　要：以徐香猕猴桃多酚为原料,对猕猴桃多酚进行分离纯化研究,探究pH、多酚浓度、乙醇洗脱浓度等对猕猴桃多酚静态吸附和解吸的影响。结果表明,最佳纯化工艺条件为多酚浓度2mg/mL、乙醇洗脱浓度90%、上样量350 mL、洗脱液体积200 mL,此时树脂对多酚吸附率最大,在此条件下纯化后猕猴桃多酚纯度为70.92%。红外光谱分析结果表明:猕猴桃多酚有多元酚类基团。而HPLC分析表明猕猴桃多酚含有7种标准酚类物质,且表儿茶素含量最高。The separation and purification of kiwifruit polyphenols has been studied with Xuxiang kiwifruit polyphenols as raw materials.The effects of pH,polyphenol concentration and ethanol elution concentration on the static adsorption and desorption of kiwifruit polyphenols were investigated.The results showed that the optimum purification conditions were 2 mg/mL of polyphenol,90%of ethanol elution concentration,350 mL of sampling amount,and 200 mL of eluent volume.At these conditons,the adsorption rate of polyphenols by resin was the largest and the purity of kiwifruit polyphenols was 70.92%.Infrared spectroscopy results indicated that the kiwifruit polyphenols had polyphenols groups.HPLC analysis showed that kiwifruit polyphenols contained seven standard phenolic substances with the highest epicatechin content.

关 键 词：猕猴桃多酚 大孔树脂 纯化工艺 组分分析 

分 类 号：TS202.3[轻工技术与工程—食品科学]