应用基因芯片技术检测食源性腹泻致病大肠杆菌  

Detection of pathogenic Escherichia coli in food borne diarrhea by gene chip technology

作　　者：刘莹[1] LIU Ying(Langfang City Center for Disease Control and Prevention,Microbiology Inspection Division,Langfang 065000,China)

机构地区：[1]廊坊市疾病预防控制中心,微生物检验科,廊坊065000

出　　处：《食品安全质量检测学报》2020年第3期915-919,共5页Journal of Food Safety and Quality

基　　金：廊坊市科技支撑计划项目(2018013083).

摘　　要：目的基因芯片技术在食源性致病大肠杆菌检测中的建立与应用。方法对菌株进行预处理,通过溴化十六烷基三甲铵(cetyltrime thylammonium ammonium bromide,CTAB)方法提取细菌中的基因组DNA并去除其中的杂质,利用提取的基因组设计引物序列和探针序列,设计完成后对致病大肠杆菌致病大肠杆就基因芯片进行杂交与洗涤,使用扫描仪对处理过的基因芯片进行扫描实现食源性致病大肠杆菌的检测。结果在病原菌按不同程度稀释的情况下,与传统的病原分离鉴定检测方法相比,将基因芯片技术应用在食源性致病大肠杆菌致检测中,能够明显的检测出荧光标记的病原菌。结论该方法的灵敏度更高,可以解决传统的致病大肠杆菌检测方法灵敏度较低,病原处于较低的感染状态时难以检出的问题。Objective To Establish and apply on gene chip technology in the detection of foodborne pathogenic Escherichia coli.Methods The strain was pretreated,genomic DNA of the bacteria was extracted by CTAB method and the impurities were removed.The extracted genome was used to design the primer sequence and the probe sequence.After design,the Escherichia coli pathogen gene chip was hybridized and washed,and the scan was performed.Scanning processed gene chip with a scanner to detect the pathogenic bacteria of the food-borne Escherichia coli.Results When the pathogens were diluted to varying degrees,compared with the traditional pathogen isolation and identification method,the detection method of the food-borne Escherichia coli pathogen using gene chip technology could detect the fluorescently labeled pathogens obviously.Conclusion This method has higher sensitivity and can solve the problems that the traditional method of detecting pathogenic Escherichia coli is low in sensitivity and difficult to detect when the pathogen is in low infection state.

关 键 词：基因芯片 食源性病原菌 大肠杆菌 病菌检测 

分 类 号：R446.5[医药卫生—诊断学]