YAP抑制剂Verteporfin对食管癌细胞存活和同质黏附能力的影响  

Effects of YAP inhibitor Verteporfin on proliferation and homogeneous adhesion of esophageal cancer cells

作　　者：王秀丽[1] 李婷[1] 杨子怡 李鑫婷 赵蓉[1] 赵欣冉 杨佳程 李晓钟[2] 王晓霞[1] WANG Xiuli;LI Ting;YANG Ziyi;LI Xinting;ZHAO Rong;ZHAO Xinran;YANG Jiacheng;LI Xiaozhong;WANG Xiaoxia(Department of Biochemistry and Molecular Biology,Shanxi Medical University,Jinzhong 030600,China;Department of Infectious Diseases,Shanxi Provincial People′s Hospital,Taiyuan 030012,China)

机构地区：[1]山西医科大学生物化学与分子生物学教研室,晋中030600 [2]山西省人民医院感染科,太原030012

出　　处：《生命的化学》2023年第2期296-302,共7页Chemistry of Life

基　　金：山西省自然科学基金项目(202103021224385)

摘　　要：为探讨Yes相关蛋白(Yes-associated protein,YAP)抑制剂Verteporfin(VP)对食管癌细胞存活和同质黏附(即细胞间黏附)能力的影响,采用噻唑蓝(thiazolyl blue tetrazolium bromide,MTT)比色法检测了不同浓度的VP对食管癌细胞KYSE150和KYSE30存活率的影响。利用细胞聚集实验、细胞分离实验检测了VP处理后对KYSE150和KYSE30细胞同质黏附能力的影响。MTT比色法实验结果显示,VP对食管癌细胞生存有抑制作用,且抑制作用随药物浓度增大而增强;细胞聚集实验和细胞分离实验结果显示,与未经药物处理的细胞相比,VP处理后的细胞聚集的团块数量较少、体积较大、细胞的分离程度小,且浓度越大越显著。以上结果提示,细胞同质黏附能力显著增强,且呈浓度依赖性。VP能抑制食管癌细胞存活,增强食管癌细胞同质黏附能力,为VP可能成为治疗食管癌的新型药物奠定了理论基础。In order to investigate the effect of Yes-associated protein(YAP)inhibitor Verteporfin(VP)on the proliferation and homogeneous adhesion(cell-to-cell adhesion)of esophageal cancer cells,thiazolyl blue tetrazolium bromide(MTT)based colorimetric method was used to detect the effect of different concentrations of VP on the proliferation of esophageal cancer cells KYSE150 and KYSE30.Cell aggregation and cell detach experiments were used to detect the effect of VP treatment on the homogeneous adhesion of KYSE150 and KYSE30 cells.MTT experiment showed that the VP had an inhibitory effect on the proliferation of esophageal cancer cells,and the inhibitory effect was enhanced with the increase of drug concentration.The results of cell aggregation and cell detach experiments showed that compared with untreated cells,the aggregated clumps of cells treated with VP were smaller in number,larger in size,and smaller degree of cell separation.With the higher of the concentration,the more significant it was.It suggested that the cell homogeneous adhesion ability was significantly enhanced in a concentration-dependent manner.VP can inhibit the proliferation of esophageal cancer cells and enhance the homogeneous adhesion of esophageal cancer cells,which lays a research foundation for VP as a new drug targeting esophageal cancer.

关 键 词：Yes相关蛋白抑制剂 VERTEPORFIN 同质黏附能力 食管癌 

分 类 号：R735.1[医药卫生—肿瘤]