Anti-cancer activity of an ethyl-acetate extract of the fruits of Terminalia bellerica(Gaertn.)Roxb.through an apoptotic signaling pathway in vitro  

作　　者：Shi Li Ting Ye Linjin Liang Wenyi Liang Ping Jian Kun Zhou Lanzhen Zhang 

机构地区：[1]School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China

出　　处：《Journal of Traditional Chinese Medical Sciences》2018年第4期370-379,共10页中医科学杂志（英文）

基　　金：supported by the National Natural Science Foundation of China:Surface Project(81274187);Longitudinal Research Project of the Beijing University of Chinese Medicine(2020072120043).

摘　　要：Objective:To investigate the biochemical constituents of the fruits of Terminalia bellerica(Gaertn.)Roxb.(hereafter termed T.bellerica)and estimate the anti-cancer activity of different polar extracts.Methods:To rapidly screen and identify the biochemical constituents of ethyl acetate(EA)extracts of T.bellerica,ultra performance liquid chromatography-electrospray ionization/mass spectrometry(UPLC-ESI-MSn)was done.The CellTiter-BlueTM cell-viability assay was used to ascertain the anti-cancer activity of different polar extracts in 10 human cancer cell lines.Results:Forty polyphenols of the EA extract of T.bellerica were characterized tentatively.The EA extract exhibited significant anti-cancer activity against ZR-75-1 cells(half-maximal inhibitory concentration=27.33(0.98)μg/mL)and Colo-205 cells(39.65(2.99)μg/mL)in vitro.Treatment of ZR-75-1 cells with 20 and 60μg/mL of the EA extract elicited dosedependent apoptosis percentages at an early stage of 17.58(0.74)%and at a late stage of 29.20(1.22)%;Colo-205 cells at the same concentration of EA extract had values of 21.33(1.03)%and 40.55(0.34)%,respectively.Western blotting suggested that ZR-75-1 and Colo205 cells treated with the EA extract showed a similar increasing tendency for expression of cleaved anti-poly adenosine diphosphate ribose polymerase I.Conclusion:We identified a total of 40 chemical constituents,of which 11 were first obtained from the Terminalia Linn.genus using UPLC-ESI-MSn.Meanwhile,we observe that the EA extract of T.bellerica possesses anti-cancer activity,especially against breast and colon cancers.

关 键 词：TERMINALIA bellerica ANTI-CANCER activity UPLC-ESI-MSn CellTiter-blue 

分 类 号：R73[医药卫生—肿瘤]