Transcription and Secretion of Interleukin-1β and HMGB1 in Keratinocytes Exposed to Stimulations Mimicking Common Inflammatory Damages  

作　　者：Xue-Cui Wei Yu-Jie Chen Fang-Yuan Long Shan-Shan Yu Song Xu Xu Chen 

机构地区：[1]School of Public Health,Nanjing Medical University,Nanjing,Jiangsu 211166,China [2]Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs,Hospital for Skin Diseases(Institute of Dermatology),Chinese Academy of Medical Sciences&Peking Union Medical College,Nanjing,Jiangsu 210042,China [3]Key Laboratory of Basic and Translational Research on Immune-Mediated Skin Diseases,Hospital for Skin Diseases(Institute of Dermatology),Chinese Academy of Medical Sciences&Peking Union Medical College,Nanjing,Jiangsu 210042,China

出　　处：《International Journal of Dermatology and Venereology》2023年第3期121-129,共9页国际皮肤性病学杂志（英文）

基　　金：supported by grants from the National Natural Science Foundation of China(81972952,81872531,82173438,and 82273550);Nanjing Incubation Program for National Clinical Research Center(2019060001);CAMS Innovation Fund for Medical Sciences(2021-1-I2M-059,2017-I2M-1-017);Jiangsu Province Foundation(LGY2018095,WSW-016);PhD Programs Foundation of Ministry of Education of China(No.20131106120046)

摘　　要：Objective:Interleukin-1β(IL-1β)and high-mobility group box 1(HMGB1)are widely known damage-associated molecular patterns(DAMPs).However,their expression and secretion in different skin diseases,especially in inflammatory skin disorders,remain to be further elucidated.This study was performed to explore and compare the transcriptional and secretory levels of IL-1β and HMGB1 in keratinocytes under 3 types of stimulation:ultraviolet B(UVB)irradiation;co-stimulation by tumor necrosis factor-α(TNF-α)and interferon-γ(IFN-γ)(simulation of T helper 1 cell inflammatory challenge);and psoriasis-like stimulation by M5,a mixture of 5 proinflammatory cytokines.Methods:We used quantitative reverse-transcription polymerase chain reaction to determine the transcription levels of IL-1β and HMGB1.Western blotting and enzyme-linked immunosorbent assay were used to detect the secretion levels of IL-1β and HMGB1.The results were statistically analyzed by t test.Results:A rapid transcriptional and secretory response of IL-1β from keratinocytes occurred in all 3 types of stimulation mimicking common inflammatory environments(P<0.05).Transcription of HMGB1 was inhibited in all 3 types of stimulation(P<0.05),but secretion was increased after exposure to UVB irradiation and co-stimulation by TNF-α and IFN-γ(P<0.05).We observed no change in the secretion level of HMGB1 after treatment with M5(P=0.196).Conclusion:IL-1β is a critical cytokine for the immunomodulatory functions of keratinocytes in inflammatory responses.In this study,keratinocytes restrained transcription of HMGB1 when the secretion of HMGB1 was induced in certain stimulations(eg,by UVB exposure or stimulation by TNF-α and IFN-γ).

关 键 词：damage-associated molecular patterns INTERLEUKIN-1Β high-mobility group box 1 KERATINOCYTES inflammatory skin disorders 

分 类 号：R285.5[医药卫生—中药学]