肺鳞癌患者线粒体DNA编码区序列突变研究  

The mutation in coding region of mitochondrial DNA in patients with squamous cell carcinoma of the lung

作　　者：刘丽红[1] 胡义德[2] 

机构地区：[1]中国人民解放军白求恩国际和平医院胃肠科,石家庄050082 [2]第三军医大学附属二院全军肿瘤中心

出　　处：《中国肺癌杂志》2007年第6期500-503,共4页Chinese Journal of Lung Cancer

基　　金：国家自然科学资助项目(No30080030);全军医药卫生科研基金攻关项目(No06G068)~~

摘　　要：背景与目的吸烟是肺癌发病的重要因素,但吸烟在肺癌发生发展中的机制目前尚不清楚。本研究旨在分析肺鳞癌患者外周血白细胞、癌旁组织及癌组织线粒体DNA(mitochondrial DNA,mtDNA)编码区序列的突变情况,并探讨其临床意义。方法应用改良一步法制备15例肺鳞癌患者外周血白细胞、癌旁组织及癌组织mtDNA,PCR产物直接测序,对比分析编码区序列突变情况。结果15例肺鳞癌患者癌组织mtDNA编码区中,有11例(73.33%)发生突变,共发现18个突变,其中10例患者有长期吸烟病史。结论肺鳞癌患者mtDNA编码区突变可能与吸烟密切相关。Background and objective Smoking plays an important role in the development of carcinoma of human lung,but the mechanism is unknown.The objective of this study is to investigate the mutation in the coding region of mitochondrial DNA(mtDNA) in patients with squamous cell carcinoma of the lung and to explore its significance in carcinogenesis.Methods White blood cells,pericarcinomatous tissues and cancer tissues were obtained from 15 cases of lung squamous cell carcinoma and mtDNA was extracted by one step method.The part of coding region fragments was amplified by PCR.Mutations were determined by DNA sequencing.Results Fifteen pairs of matched pericarcinomatous tissues and cancer tissues were screened for mutation in coding regions,18 mutations were detected in the coding region of mtDNA in 11 patients,of which 10 cases were smokers.Conclusion The mutations of the coding region of mtDNA in squamous cell carcinoma of the lung may be correlative with smoking.

关 键 词：肺鳞癌 线粒体DNA 编码区 吸烟 突变 

分 类 号：R734.2[医药卫生—肿瘤]