它莫西芬诱导乳腺癌MCF-7细胞自噬的比较蛋白质组学研究  

Proteomics Analysis of Tamoxifen-induced Autophagic MCF-7 Cells

作　　者：娄雅欣[1] 钟丽君[1] 杨彬 陈丽娜[2] 刘丹 邹霞娟 彭嘉柔 

机构地区：[1]北京大学医药卫生分析中心,北京100083 [2]北京大学人类疾病基因研究中心,北京100083

出　　处：《分析测试学报》2007年第z1期78-79,共2页Journal of Instrumental Analysis

摘　　要：The differentially expressed proteins between normal and tamoxifen-induced autophagic MCF-7 cells were studied by 2-DE combining with mass spectrometric analysis.Totally,there were 668±99 and 550±97 protein spots detected in the 2-DE maps from normal and autophagic MCF-7 cells respectively,among which 5 changed protein spots were identified by the Q-TOF MS/MS and database search.The differentially expressed proteins between normal and tamoxifen-induced autophagic MCF-7 cells were studied by 2-DE combining with mass spectrometric analysis.Totally,there were 668±99 and 550±97 protein spots detected in the 2-DE maps from normal and autophagic MCF-7 cells respectively,among which 5 changed protein spots were identified by the Q-TOF MS/MS and database search.

关 键 词：PROTEOMICS Two dimensional gel electrophoresis Mass spectrometry AUTOPHAGY 

分 类 号：O657-55[理学—分析化学]