微囊藻对银鲫生长和消化酶活性的影响及毒素积累  

Effects of toxic microcystis on the growth,activities of digestive enzymes and accumulation of microcystin in Carassius auratus

作　　者：姚雁鸿[1] 余来宁[2] 何文辉[3] 李谷[1] 罗晓松[1] 

机构地区：[1]中国水产科学研究院长江水产研究所 [2]江汉大学医学与生命科学院,湖北武汉430056 [3]上海水产大学生命科学与技术学院,上海200090

出　　处：《中国水产科学》2007年第6期969-973,共5页Journal of Fishery Sciences of China

基　　金：农业部农业结构调整项目(05-08-2A);中国水产科学研究院淡水生态与健康养殖重点开放实验室第五期开放课题

摘　　要：研究了淡水常见养殖品种银鲫(Carassius auratus)在有毒微囊藻环境条件下的生长和消化酶活性以及微囊藻毒素在鱼体内的积累情况。实验分为添加有毒微囊藻的实验组和不添加微囊藻的对照组2组,每组设3个平行,每个平行10尾银鲫,实验周期42 d。结果表明,有毒微囊藻能够极显著的抑制银鲫的生长(P<0.01),添加微囊藻实验组的增重率为38.6%,不添加微囊藻的增重率为87.70%;有毒微囊藻能显著降低银鲫的消化酶活性(P<0.05),添加微囊藻的实验组的银鲫的肠、肝胰脏蛋白酶活性和肠、肝胰脏淀粉酶活性分别是对应不添加微囊藻的对照组活性的60.6%、71.3%、65.4%、68.2%;而且毒素会最终在银鲫体内积累,并可通过食物链传到人类,从而对人类构成潜在的风险。因此,养殖水体微囊藻水华应该被引起足够重视。Two groups of aquaculture system were designed to evaluate the toxic effect of Carassius auratus.One was experimental group,in which the toxic microcystis was introduced and the density of Microcystis was maintained at 1×107-3×107 ind/mL.Another was control group,in which no toxic microcystis was introduced.Each group containsed three replicates.Every replicate contained 10 Carassius auratus.The experiments were conducted in six aquaria.Experiment period was 42 d.The results demonstrated that toxic microcystis environment had significant suppressive effects on growth(P<0.01) and activities of digestive enzymes(P<0.05) in Carassius auratus.The growth gain rate of C.auratus of experiment groups was 38.6%,while that of control groups were 87.7%.The activities of protease in the intestine and hepatopancreas in experiment groups were 60.6% and 71.3% of those in control,respectively.Meanwhile,the activities of amylase in the intestine and hepatopancreas of experiment groups were 65.4% and 68.2% of those in control,respectively.The microcystin could accumulate in Carassius auratus body.The fish act as potential vectors to transfer the microcystin to people and may be a hazard to people's health.So more regard should be paid to the microcystis bloom in fish aquaculture.

关 键 词：银鲫 微囊藻 生长 消化酶活性 微囊藻毒素 积累 

分 类 号：X171.5[环境科学与工程—环境科学]