柱前衍生高效液相色谱法测定红牛维生素功能饮料中牛磺酸含量  

Taurine in RedBull Super Vitamin Drinks Determined by Pre-column Derivatization and HPLC

作　　者：黄岛平[1] 陈秋虹[1] 劳燕文[1] 

机构地区：[1]广西分析测试中心,广西南宁530022

出　　处：《广西科学院学报》2006年第z1期419-421,共3页Journal of Guangxi Academy of Sciences

摘　　要：用邻苯二甲醛(OPA)柱前衍生高效液相色谱法,运用C18色谱柱为固定相,甲醇+0.05mol/L NaAc溶液(45+55)为流动相,检测波长330nm,测定红牛维生素功能饮料的牛磺酸.结果衍生化反应迅速,分离效果好,在10～50μg/ml的浓度范围内,牛磺酸色谱峰面积与浓度之间的线性相关系数为0.9999,加标回收率为97.48%～101.0%,RSD为2.5%.Taurine in RedBull Super Vitamin Drinks was determined by OPA pre-column derivatization and High Performance Liquid Chromatography(HPLC),using C_(18) column as fixed phase,methyl alcohol+0.05mol/L NaAc as mobile phase(45+55 by volume),with wavelength 330nm.The separation effect is satisfactory;derivatization reaction is rapid;the linear correlation coefficients of peak areas and concentration is 0.9999 in the range of 10~50g/ml respectively;the recoveries of the Taurine were 97.48%~101.0%,RSD were 2.5%.This method is accurate and suitable for quality control of Tongluoxiaru Koufuye.

关 键 词：高效液相色谱法 牛磺酸 饮料 

分 类 号：O657.72[理学—分析化学]