Identification and significance of differential proteins in A549 cells transfected with HLCDG1  

作　　者：ZOU Fei-yan XIE Hai-long ZENG Ping-yao CHEN Zhu-chu LI Feng XIAO Zhi-qiang FENG Xue-ping ZHANG Peng-fei YANG Hai-yan HU Wei YU Yan-hui OUYANG Yong-mei 

机构地区：[1]Laboratory of Cell Biology, Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha 410078, China [2]Institute for Reproductive Immunology Research, Jinan University, Guangzhou 510632, China Department of Pathology of Medical College, Nanhua University, Hengyang 421001, China Laboratory of Cell Biology, Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha 410078, China Laboratory of Cell Biology, Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha 410078, China [3]Medical Research Center of Xiangya Hospital, Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Central South University, Changsha 410008, China Laboratory of Cell Biology, Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha 410078, China Medical Research Center of Xiangya Hospital, Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Central South University, Changsha 410008, China Medical Research Center of Xiangya Hospital, Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Central South University, Changsha 410008, China Medical Research Center of Xiangya Hospital, Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Central South University, Changsha 410008, China Laboratory of Cell Biology, Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha 410078, China [4]Medical Research Center of Xiangya Hospital, Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Central South University, Changsha 410008, China Laboratory of Cell Biology, Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha 410078, China [5]Medical Research Center of Xiangya Hospital, Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Central South University, Changsha 410008, China Laboratory of Cell Biology, Cancer Research Institute, Xiangya School of Medicine, Central South Un

出　　处：《Journal of Central South University of Technology》2005年第z1期337-342,共6页中南工业大学学报（英文版）

基　　金：Projects(30000074, 30471954) supported by the National Natural Science Foundation of China; project(2003034467)supported by the Postdoctoral Science Foundation of China

摘　　要：HLCDG1, which locates in chromosome 5q33, is a novel gene cloned recently. The HLCDG1 expression was significantly down regulated in the primary lung carcinoma. It was previously studied that HLCDG1 acted like a tumor suppressor gene. In this paper, proteomics studies were performed to analyze the proteomic expression patterns in the HLCDG1-transfected human lung carcinoma cell line (A549-HLCDG1) and in the control vector-transfecred human lung carcinoma cell line (A549-vector). Employing two dimensional gel eleetrophoresis (2DE), the global pattern of protein expressions in A549-HLCDG1 human lung adenocarcinoma cell line expressing stably HL-CDG1 gene were compared with those of control A549-vector cell line to generate a differential protein expression catalog. Forty-two differentially expressed proteins were screened. Thirteen differential proteins were identified by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS), which were 6 upregulated (MSH5, MOD, MDH precursor, ETFβ, Prxd Ⅵ and JM23) and 7 downregulated (PLC-δ1, hnRNPA2,hnRNPB1, TIM, TCTP, nm23H-1 and PrxdⅤ) proteins in A549-HLCDG1 cells compared to control A549-vector cells. The above identified proteins were involved in energy metabolism, transcription regulation, antioxidation,cell cycle, metastasis, DNA methylation and mismatch repair. Therefore, these differential expression proteins by HLCDG1 transfection may play some important roles for investigation of the biochemical basis of growth suppression of HLCDG1 gene in lung carcinoma cells A549. Further understanding of this data base may provide valuable resources for the developing novel diagnostic markers and therapeutic targets of lung cancer.

关 键 词：HLCDG1 gene LUNG carcinoma cell two-dimensional GEL ELECTROPHORESIS mass SPECTROMETRY PROTEOMICS 

分 类 号：R734.2[医药卫生—肿瘤]