肝癌及肝硬化患者血清HBV X基因检测及序列分析  

Expression and Nucleotide Sequence Analysis of Hepatitis B Virus X Gene in Serum of Hepatocellular Carcinoma and Cirrhotic Patients

作　　者：蒋向荣[1] 陈治新[1] 李丹[1] 王小众[1] 

机构地区：[1]福建医科大学附属协和医院,福建福州350001

出　　处：《中西医结合肝病杂志》2003年第S1期8-10,共3页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：福建省肝癌攻关课题分题(99-Z-14);福建省科技课题(99-Z-162)资助

摘　　要：目的:了解肝癌及肝硬化患者血清HBV X基因的表达及其核苷酸序列的变异情况。方法:采用酚-氯仿抽提法抽提患者血清DNA,聚合酶链反应扩增血清HBV X基因,琼脂糖电泳观察结果,并对PCR产物行序列分析。结果:HBV X基因在肝癌患者血清的检出率为32％(16/50),在肝硬化患者血清的检出率为46％(23/50)。经琼脂糖电泳及序列分析证实为HBV X基因。16例肝癌及15例肝硬化患者X基因序列分析显示患者的X基因均存在不同程度的点突变(1～13bp),未发现固定的突变部位与序列。结论:肝癌及肝硬化患者血清HBV X基因存在不同程度点突变,但无特异性突变序列。Objective: io explore the expression and variety of nucleotide sequence of hepatitis B virus X gene in serum of hepatocellular carcinoma and cirrhotic patients. Methods: DNA was extracted from the serum by phenol-chloroform extracted method and HBV X gene was amplified by polymerase chain reaction. The PCR product was separated in agarose gel and analyzed by automatic sequencing. Results: Serum HBV X gene was found in 32% of HCC patients (16/50) and 46% of cirrhotic patients (23/50). Automatic sequencing an...

关 键 词：肝肿瘤 肝硬化 乙肝病毒 X基因 

分 类 号：R735.7[医药卫生—肿瘤]