一株产黏特性的乳酸菌及其应用研究  

Study on a lactic acid bacteria with sticky characteristics and its application

作　　者：王艳萍[1] 李超[1] 张玢[1] Zaheer Ahmed 

机构地区：[1]天津科技大学食品工程与生物技术学院,天津300457

出　　处：《中国食品添加剂》2009年第1期91-95,81,共6页China Food Additives

基　　金：天津市应用基础及前沿技术研究计划项目(项目编号08JCYBJC01900)

摘　　要：利用苯酚硫酸法从分离自西藏Kefir粒的11株乳酸菌中筛选得到了一株胞外多糖(EPS)产量较高的乳酸菌KF5,经生理生化实验及分子遗传学鉴定该菌株为植物乳杆菌(Lactobacillus plantarum)。生物学特性表明KF5具有较好的耐酸性以及一定的胆盐耐受力,该菌株具有较强的产胞外多糖的能力。将添加有KF5的发酵乳与传统发酵乳相比较,其发酵乳的特性(产酸量、乳清析出率、黏度)良好,可作为取代植物或动物增稠剂使用。The exopolysaccharide content was tested on 11 strains of lactic acid bacteria isolated from Tibet kefir by the phenol-sulphate acid method.KF5 strain was screened for its better exopolysaccharide producing ability.KF5 strain was identified as Lactobacillus plantarum by its morphology,biochemical identification and sequence analysis of 16S rDNA.Study on the biological characters of KF5 strain shows that KF5 strain has a good acid and bile salt tolerance.Comparing fermented milk adding KF5 strain with tradit...

关 键 词：EPS 筛选 鉴定 特性 

分 类 号：TS201.3[轻工技术与工程—食品科学]