超声集成丙醇-硫酸铵双水相体系从苦荞麦苗中提取总黄酮及其抗氧化活性研究  

Study on Extraction of Total Flavonoids from Tartary Buckwheat Seedling Using Ultrasonic Coupled with Propyl Alcohol-Ammonium Sulfate Aqueous Two-phase Separation and Antioxidant Activity of Total Flavonoids Extract

作　　者：高云涛[1,2] 李干鹏[1,2] 李正全[2] 唐光阳[1,2] 张宝通[1] 

机构地区：[1]云南民族大学化学与生物技术学院,云南昆明650031 [2]云南民族大学应用化学研究所,云南昆明650031

出　　处：《食品科学》2009年第2期110-113,共4页Food Science

基　　金：云南省社会发展科技计划项目(2007B148M);云南省教育厅科学基金项目(06Y195C);国家民委-教育部共建民族药资源化学重点实验室开放基金项目(MJY07010)

摘　　要：本实验利用超声提取集成丙醇-硫酸铵双水相体系对苦荞麦苗中总黄酮进行提取分离,研究丙醇-硫酸铵双水相的形成和提取条件对总黄酮得率的影响。结果表明,本方法可有效地从苦荞麦苗中提取总黄酮,提取率为1.86%,提取物中总黄酮含量为19.3%,明显高于回流提取法。提取物对脂质过氧化具有良好的抑制作用,最大抑制率为68.2%,方法操作简便、条件温和,环境友好。The ultrasonic extraction technology coupling with propyl alcohol-ammonium sulfate aqueous aqueous two-phase separation for extracting total flavonoids from tartary buckwheat seedling was studied. The results showed that under the optimum conditions,the extraction rate of total flavonoids is 1.86 % and their content in extract is 19.3 %,which is much higher than that extracted by circumfluence of solvent. Moreover,the total flavonoids extract could singificant1y inhibit lipid peroxidation (LPO) induced by ·...

关 键 词：超声波提取 双水相分离 苦荞麦苗 总黄酮 抗氧化 

分 类 号：TQ461[化学工程—制药化工]