中国林蛙蛙皮肽抗菌性质及其机理的研究  

Study on Antimicrobial Activity and Mechanism of Peptide from Rana chensinensis Skin

作　　者：刘红玉[1] 崔洪斌[2] 许岩[1] 

机构地区：[1]东北农业大学食品学院,黑龙江哈尔滨150030 [2]哈尔滨医科大学公共卫生学院,黑龙江哈尔滨150086

出　　处：《食品科学》2009年第5期64-67,共4页Food Science

基　　金：黑龙江省教育厅科学技术研究项目(11511142)

摘　　要：本实验对从中国林蛙的皮肤中提取的蛙皮肽的抗菌性质及其机理进行了研究。结果表明,该蛙皮肽抗菌谱较广,对革兰氏阴性菌、革兰氏阳性菌、酵母菌都有较好的抗菌效果,其抗菌活性具有很强的热稳定性,且在酸性条件下抗菌活性强,当pH值大于8时丧失抗菌活性。对经蛙皮肽处理的枯草杆菌作电镜扫描检查,发现细菌细胞壁出现破损或穿孔,而对照组细菌壁结构完整,表明该蛙皮肽的抗菌机制是通过破坏细菌细胞壁,使细胞质外流而致菌体死亡。The peptide from Rana chensinensis skin has broad antibacterial spectrum, and may inhibit Gram-negative, Gram- positive bacteria and yeast. The antibacterial activity also shows fairly strong thermal and acid stabilities. But when pH value is above 8, the antibacterial activity disappears. Compared with the intact cell wall of Bacillus subtilis in the control group, the broken and perforated phenomena of cell wall of Bacillus subtilis treated with the peptide was detected with scanning electron microscopy (...

关 键 词：中国林蛙 蛙皮肽 抗菌活性 

分 类 号：Q51[生物学—生物化学]