鲑鱼降钙素与降钙素基因相关肽融合基因植物表达载体的构建  

Construction of Plant Expression Vector of Salmon Calcitonin (sCT) and Calcitonin Gene-related Peptide (CGRP) Fusion Gene

作　　者：余琼[1,2] 王超 李荣田[1,2] 平文祥[1,2] 孙怡宁[1,2] 李洪丽[1,2] 

机构地区：[1]黑龙江省高等学校分子生物学重点实验室 [2]黑龙江大学生命科学学院,黑龙江哈尔滨150080

出　　处：《食品科学》2009年第5期126-129,共4页Food Science

基　　金：黑龙江省教育厅2007年科学技术研究面上项目(11521223)

摘　　要：根据基因工程操作方法和植物偏爱密码子原则,设计了鲑鱼降钙素与降钙素基因相关肽融合基因,并在融合基因前端加上AMVRNA45'-UTR序列和6×His标签,在融合基因后边加上一个甘氨酸。通过SacI和SmaI双酶切pUC(AMV/CGRP/msCT)获得融合基因,用T4DNA连接酶连到植物表达载体pBI121上,构建重组质粒pBIAMV/CGRP/msCT,再转化到E.coliDH5α中保存。通过DNA测序验证表达载体构建成功。The fusion gene of sCT and CGRP was designed according to the principles of genetic engineering method and the bias codon used by plant. An AMV RNA4 5 -UTR sequence and a 6 × His tag were added before the gene, and the Gly was added at the end of the fusion gene. The fusion gene was obtained from pUC(AMV / CGRP / msCT) by Sac I and Sma I digestion, then cloned into plant expression vector pBI121 by T4 DNA ligase to construct recombinant plasmid pBI AMV / CGRP / msCT, and transformed into E.coli DH5α to be k...

关 键 词：鲑鱼降钙素 降钙素基因相关肽 基因工程 融合表达 高血压 骨质疏松 

分 类 号：Q943.2[生物学—植物学]