酸性环境中木聚糖酶高产菌株的筛选及鉴定  

Screening and identification of strain with high producing acidic xylanase from acidic entrionment

作　　者：韩锐[1] 吴鹏[1] 曹瑜[1] 马根[1] 马小珍[1] 马志鹰[1] 许悟[1] 曹毅[1] 乔代蓉[1] 

机构地区：[1]四川大学生命科学学院四川省生物信息与代谢工程共享实验平台,成都610065

出　　处：《四川大学学报（自然科学版）》2009年第2期520-524,共5页Journal of Sichuan University(Natural Science Edition)

基　　金：国家科技基础条件平台(2005DKA21208);四川省科技厅(04NG002-006-1;06PT-07;07PT-03);四川省杰出青年基金(05ZQ026-029)

摘　　要：从pH 5.0的酸性土壤中筛选出一株木聚糖酶高产茵株A4,菌体固态发酵产酶条件优化表明,最佳发酵培养基配方为:麸皮37.79%,玉米芯9.10%,NH_4NO_3 0.51%,MnSO_41.60%,水50%,接种量2.0%,最适发酵温度28～32℃,发酵培养48～52h,木聚糖酶活力最高达到750 U/g(碳源)。该菌株所产木聚糖酶的最适pH为4.8,比野生黑曲霉的pH值低。通过生长形态和分子生物学方法相结合的鉴定该菌株为黄曲霉。A strain A4 with high xylanase yield was isolated from acidic entironment with pH 5.0. By solid -state fermentation to produce xylanase, the optimum medium component was brain 37.79%,corncob 9.10%, NH_4NO_3 0.51%, MnSO_4 1.60%,water 50%. The optimum inoculum was 2%, the temperature was 28～32℃,and the cultivated time was 48～54 hours. Xylanase activity could reach 750 U/g. The optimum pH of xylanase activity is 4.5 which lower than xylanase produced by Aspergillus niger. The strain is identified as Aspergillus flavus through the methods of combing modality and moleculebiology together.

关 键 词：木聚糖酶 菌种鉴定 发酵优化 酶学性质 

分 类 号：Q93[生物学—微生物学]