bFGF发酵工艺参数正交优化试验  

Study of the technology on bFGF fermentation

作　　者：王士杰[1] 秦立屿[1] 于忠喜[2] 

机构地区：[1]长春工业大学生物工程学院,吉林长春130012 [2]长春金赛药业有限责任公司,吉林长春130012

出　　处：《吉林工程技术师范学院学报》2007年第9期41-42,共2页Journal of Jilin Engineering Normal University

摘　　要：通过正交实验研究了bFGF发酵的各主要影响因素对于最终产物的数量和纯度的关系,从而确定发酵的最适工艺条件。结果表明当温度控制在37℃,pH值7.0,发酵时间10小时,IPTG诱导浓度为100μg/mL,消泡剂为5 mL/L时,调节溶解氧在40-60%之间对于整个发酵过程是非常有利的。By means of orthogonal test,the effects of factors on bFGF fermentation were studied and the best available condations were determined.The results show that condations that temperature 37℃,pH7.0,the induction of IPTG100μg/mL,antifoam5mL/L,the time of 10 hours and so on,E.coli lives in best state,the expression of plasmid is best and proteins get large amount.

关 键 词：BFGF 重组DNA大肠杆菌 发酵 表达 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]