Various tolerances to arsenic trioxide between human cortical neurons and leukemic cells  

作　　者：ZHOU Jin1,MENG Ran1,2,SUI Xinhua2,LI Wenbin3 & YANG Baofeng1 1.The First Hospital of Harbin Medical University,Harbin 150001,China 2. Shijitan Hospital,The Ninth Clinical Medical College of Peking University,Beijing 100038,China 3. Xuanwu Hospital,Capital University of Medical Sciences,Beijing 100053,China 

出　　处：《Science China(Life Sciences)》2006年第6期567-572,共6页中国科学（生命科学英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant No.30370507);the Key Projects Foundation of Harbin Science and Technology Commission(Grant No.2003AA9CS188-12).

摘　　要：Arsenic trioxide (As2O3) is very effective for treatment of acute promyelocytic leukaemia (APL) but little can pass through the blood-brain-barrier (BBB),which limits its use in the prevention and treatment of central nervous system leukaemia (CNSL). Before creating a non-invasive method to help As2O3 ’s access,the safe and effective therapeutic concentration of As2O3 in the CNS ought to be known. The changes of apoptosis biomarkers,[Ca2+]i and PKC activity of both leukaemia cells and human cortical neurons,were monitored before and after being treated with As2O3 in vitro with laser confocal microscopy and Western blot. NSE concentration,the neuron invasive biomarker,was monitored by enzyme immunoassay (NSE-EIA). This study revealed that cortical neuron was more tolerable to As2O3 compared to NB4. 1.0 μmol / L As2O3 showed little influence on cortical neuron but effectively promoted apoptosis and induced differentiation of NB4.Arsenic trioxide (As2O3) is very effective for treatment of acute promyelocytic leukaemia (APL) but little can pass through the blood-brain-barrier (BBB),which limits its use in the prevention and treatment of central nervous system leukaemia (CNSL). Before creating a non-invasive method to help As2O3 ’s access,the safe and effective therapeutic concentration of As2O3 in the CNS ought to be known. The changes of apoptosis biomarkers,[Ca2+]i and PKC activity of both leukaemia cells and human cortical neurons,were monitored before and after being treated with As2O3 in vitro with laser confocal microscopy and Western blot. NSE concentration,the neuron invasive biomarker,was monitored by enzyme immunoassay (NSE-EIA). This study revealed that cortical neuron was more tolerable to As2O3 compared to NB4. 1.0 μmol / L As2O3 showed little influence on cortical neuron but effectively promoted apoptosis and induced differentiation of NB4.

关 键 词：arsenicals  protein KINASE C  NEURONE specific enolase. 

分 类 号：R733.7[医药卫生—肿瘤]