豫医无毛小鼠无毛基因突变部位的定位和分析  

Orientation and analysis of Yuyi hairless mice hairless gene mutation point

作　　者：臧文巧[1] 杨璇[1] 王涛[2] 轩小燕[1] 李敏[1] 杜英[1] 

机构地区：[1]郑州大学基础医学院微生物学与免疫学教研室,郑州450001 [2]河南中医学院第一附属医院血液肿瘤科,郑州450000

出　　处：《郑州大学学报（医学版）》2009年第6期1144-1146,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家自然科学基金资助项目39970119

摘　　要：目的:研究豫医无毛小鼠(YYHL)突变的分子机制。方法:对豫医无毛小鼠和昆明小鼠的基因组DNA进行PCR扩增,对扩增片段克隆后测序,并与Musmuscnlus进行同源性分析。结果:共测出YYHL DNA序列1824bp和昆明小鼠DNA序列1816bp,其不同之处有27处,变异方式包括插入、缺失和点突变。其中在12外显子中有一无义突变(G→A),导致该部位由编码色氨酸的UGG转变为终止密码子UGA。结论:YYHL无毛性状的产生可能与基因组的变异有关。Aim:To research the hairless gene mutation of Yuyi hairless mice.Methods:Partial hairless gene of Yuyi hairless mice and Kunming mice were cloned,sequenced,and compared with Mus musculus DNA.Results:The DNA fragment of Yuyi hairless mice contained 1 824 bp,that of Kunming mice contained 1 816 bp.There were 27 differences,including insertion,deletion,and point mutation.One nonsense mutation in exon 12 resulted in the introduction of a premature termination codon UGA.Conclusion:Hairless character is probably ...

关 键 词：豫医无毛小鼠 基因 突变 

分 类 号：R-332[医药卫生]