Study on Effect of IH764-3, an Active Principle of Salviae miltiorrhizae, in Inducing Hepatic Stellate Cell Apoptosis  

作　　者：赵东强 姜慧卿 修贺明 张晓岚 

机构地区：[1]Department of Gastroenterology, Second Hospital of Hebei Medical University, Shijiazhuang 050000 [2]Central Laboratory of Norman Bethune International Peace Hospital

出　　处：《Chinese Journal of Integrative Medicine》2002年第2期126-129,共4页中国结合医学杂志（英文版）

基　　金：ThisstudywassupportedbyAdministryofSciencesandTechnique;HebeiProvince(No.0 12 76 134)

摘　　要：Objective: To explore the anti fibrotic mechanism of Salviae miltiorrhizae from the view of proliferation and apoptosis of hepatic stellate cells (HSC).Methods: IH764 3, an active principle of Salviae miltiorrhizae, was used to intervene in the cultured HSC in vitro. Cell proliferation was detected by methyl thiazolyl tetrazolium (MTT) method, and the cell apoptosis was examined by electron microscopy, flow cytometer and terminal deoxynucleotidyl transferase mediated dUTP nick end labeling method (TUNEL).Results: MTT showed that IH764 3 has obvious inhibition on the proliferation of HSC. Specific cell apoptosis figures of HSC, such as chromatin agglutination, were seen under electron microscopy in the IH764 3 treated group. By flow cytometer, it was shown that the HSC apoptosis rate in the IH764 3 treated group was higher than that in the control group, and the apoptosis inducing effect of IH764 3 was dose and time dependent. TUNEL analysis showed that the HSC apoptotsis rate was 28.3±1.5% after being incubated for 48 hrs with IH764 3, which was significantly higher than that in the control group (6.7±0.6%, P <0.05).Conclusion: IH764 3 could inhibit the proliferation of HSC and induce its apoptosis. These effects may be one of the anti fibrotic mechanisms of Salviae miltiorrhizae.Objective: To explore the anti fibrotic mechanism of Salviae miltiorrhizae from the view of proliferation and apoptosis of hepatic stellate cells (HSC).Methods: IH764 3, an active principle of Salviae miltiorrhizae, was used to intervene in the cultured HSC in vitro. Cell proliferation was detected by methyl thiazolyl tetrazolium (MTT) method, and the cell apoptosis was examined by electron microscopy, flow cytometer and terminal deoxynucleotidyl transferase mediated dUTP nick end labeling method (TUNEL).Results: MTT showed that IH764 3 has obvious inhibition on the proliferation of HSC. Specific cell apoptosis figures of HSC, such as chromatin agglutination, were seen under electron microscopy in the IH764 3 treated group. By flow cytometer, it was shown that the HSC apoptosis rate in the IH764 3 treated group was higher than that in the control group, and the apoptosis inducing effect of IH764 3 was dose and time dependent. TUNEL analysis showed that the HSC apoptotsis rate was 28.3±1.5% after being incubated for 48 hrs with IH764 3, which was significantly higher than that in the control group (6.7±0.6%, P <0.05).Conclusion: IH764 3 could inhibit the proliferation of HSC and induce its apoptosis. These effects may be one of the anti fibrotic mechanisms of Salviae miltiorrhizae.

关 键 词：Salviae miltiorrhizae hepatic stellate cell PROLIFERATION APOPTOSIS 

分 类 号：R363[医药卫生—病理学]