淡紫拟青霉培养过程中酶及可溶性蛋白的变化  

CHANGES OF ENZYME AND PROTEIN COMPONENTS DURING THE GROWTH OF THE PAECILOMYCES LILACINUS

作　　者：车建美[1] 李芳[2] 刘波[1] 

机构地区：[1]福建省农科院生物技术中心,福建福州350003 [2]福建农林大学植保学院,福建福州350003

出　　处：《武夷科学》2005年第1期1-6,共6页Wuyi Science Journal

基　　金：国家863计划项目(2002AA244031-2);福建省科技厅重大项目(2000Z031);福建省计委项目(闽计农经[2002]48号)

摘　　要：对淡紫拟青霉培养过程POD同工酶进行跟踪测定,结果表明:在淡紫拟青霉的整个液体培养过程中,共出现了4条POD同工酶酶带,其迁移率(R f)分别为0.098、0.137、0.294和0.373,总体上,培养过程POD同工酶酶带的数量呈现减少的趋势,在第3-5 d时有3条POD同工酶酶带,培养至第6 d时,酶带减少了1条,只有2条酶带,从发酵的第7-10 d都只有1条POD同工酶酶带。淡紫拟青霉培养过程β-葡萄糖苷酶活性在培养的第1-6 d时变化比较平缓,酶活的变化为7.0 u/mL-9.438 u/mL,从培养的第7 d开始β-葡萄糖苷酶活性骤然升高,达到43.875 u/mL,培养8 d时,β-葡萄糖苷酶活性达到最高值55.0 u/mL,随后,β-葡萄糖苷酶活性动态平衡状态(9 d-11 d),12 d时开始呈现逐渐下降的稳定变化,从44.438 u/mL降至18.875u/mL。淡紫拟青霉培养过程的可溶性蛋白的电泳结果表明:培养各个阶段存在着共同的蛋白质电泳条带,在1-12 d的培养过程中,蛋白质条带呈现先增多,后减少,再增多的趋势,这反映了淡紫拟青霉发酵过程中基因表达的情况。Analyzed the soluble protein from the mycelia of Paecilomyces lalicinus in the duration of liquid culturing process with SDA-PAGE,the result showed that: there were common bands in the all culturing duration.At the early duration,more of bands were produced,at the middle term,the bands decreased,but to the end of culturing duration,the bands increased.That could be deduced that the presence of soluble protein bands was a good indicator of culturing process.The POD isoenzyme from the mycelia of Paecilomyces lalicinus in the process of liquid culturing has been electrophoresis analyzed.The result showed that: there were four affinity isoenzyme bands produced on the gel.The Rf value were 0.098,0.137,0.294 and 0.373 respectively.In the general trend,the bands number decreased along with the liquid culture.In the 3-5th day of liquid culturing,there were three bands produced on the gel.In the 6th day,two bands produced,but to the lately duration(from 7-10th)only one band existed,this result showed that the patterns of isoenzyme was a sign of culturing duration.All culturing periods could produce cell-wall-degrading enzymes such as β-glucosidase,at the early term(1-6th) the activity of β-glucosidase increased gradually,from 7.0 u/mL to 9.438 u/mL.In the 7th day of culturing duration the activity grew up abruptly,from 9.438 u/mL to 43.875 u/mL,at 8th day reach 55.0 u/mL,as time goes on,the activity maintain dynamic balance.

关 键 词：淡紫拟青霉 过氧化物酶同工酶 Β-葡萄糖苷酶 可溶性蛋白质 

分 类 号：Q939.9[生物学—微生物学]