Neuregulin-1 therapy improved cardiac function and reduced ANPmRNA expression in post myocardial infarction rats with cardiac dysfunction  

作　　者：Yuanfang LI Naisheng CAI Xinghua GU Jingmin ZHOU Keqiang WANG Yunzeng ZOU Junbo GE 

机构地区：[1]Department of Cardiology,Zhongshan Hospital,Fudan University,Shanghai Cardiovascular Disease Research Institute,Shanghai 200032,China

出　　处：《Journal of Geriatric Cardiology》2005年第4期236-239,共4页老年心脏病学杂志(英文版)

摘　　要：Objective To observe the influence of neuregulin-1 on the cardiac function of post-myocardial infarction rats.Methods Left ventricular MI was created in Sprague-Dawley rats by ligation of the left anterior descending coronary.Six months after the operation,rats were evaluated with echocardiology methods.36 rats that had an infarct area and a EF around 60%were randomized into 3 groups:MI group(n=12)were injected a blank vehicle fluid intravenously for 5 days,after which they continued to be raised on standard food and water for 30 days.MI+NRG group(n=12),received NRG-110μg·kg-^(1) intravenously for 5 days,after which they continued to be raised on standard food and water for 30 days.MI+Capt group(n=12)received captopril orally(dissolved in their drinking water 2g/L)for 30days,after which tap water substituted the solution for 5 days.Final echocardiographic and hemodynamic measurements were made at the end of 1 month of therapy.Total RNA was extracted from frozen left ventricular tissues,and was reverse transcribed into firststrand PCR was performed with primers for BNP、ANP.Results Rats treated with neuregulin had a smaller LVDs(P=0.014),a betterLVEF(P=0.004),and a tendency towards less lung perfusion than untreated rats.Neuregulin decreased the expression of ANP mRNA in the ventricle(P=0.025).Conclusion Neuregulin markedly improved the cardiac function of rats that survived myocardial infarction,and decreased the expression of ANP mRNA in the ventricle.

关 键 词：NEUREGULIN-1 myocardial infarction heart failure atrial natriuetic peptide 

分 类 号：R[医药卫生]