复合诱变选育高产脂肪酶黑曲霉菌株及其固定化酶性质  

Composite Mutation Breeding of Aspergillus niger Strain with High Lipase Activity and Immobilized Lipase Properties

作　　者：孟娟[1] 曾虹燕[1] 张艳梅[1] 夏葵[1] 刘贵[1] 

机构地区：[1]湘潭大学化工学院生物技术研究所,湘潭411105

出　　处：《天然产物研究与开发》2010年第2期209-214,231,共7页Natural Product Research and Development

基　　金：湘潭市科技重点资助项目ZD20081027;教育部"大学生创新实验计划"项目081053013

摘　　要：通过硫酸二乙酯(DES)和微波复合诱变,获得遗传性状稳定的高产脂肪酶黑曲霉突变菌株CM2,酶活达174.93U/mL。对菌株CM2培养条件的优化,以橄榄油和(NH4)2SO4为最佳碳、氮源,在28℃、pH7.5的条件下,发酵CM2菌株68h,脂肪酶活为180.52U/mL。大孔树脂固定化脂肪酶在35～55℃和pH7.5～9.5之间有很好的稳定性。游离酶和固定化酶的表观失活活化能分别为52.6842kJ/mol和30.8391kJ/mol,固定化酶对温度的敏感度降低,耐受性增强。在微水相中脂肪酶催化2-辛醇和乙酸乙烯酯不对称酯交换反应中,(S)-乙酸辛酯的对映选择性高(游离酶e.e.S85.7%;固定化酶e.e.S87.7%),显示了该固定化酶在2-辛醇的手性拆分方面具有良好的应用前景。The Aspergillus niger CM2 with high lipase activity (174.93 U/mL) was obtained by combining microwave irradiation with DES.The hereditary character of the strain CM2 was stable.The culture conditions of the strain CM2 were optimized.The lipase activity was 180.52 U/mL when the strain was cultured in the medium at 28 ℃ and pH 7.5 for 68 h,where olive oil and (NH4)2SO4 were used as the carbon and nitrogen sources,respectively.Comparing with the free lipase,the immobilized lipase adsorbed on the macroporous re...

关 键 词：黑曲霉 脂肪酶 复合诱变 固定化 酶拆分 

分 类 号：Q814.2[生物学—生物工程]