Binding of EGF1 Domain Peptide in Coagulation Factor Ⅶ with Tissue Factor and Its Implications for the Triggering of Coagulation  

作　　者：梅恒 胡豫 王华芳 石威 邓君 郭涛 

机构地区：[1]Institute of Hematology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology [2]Targeted Biotherapy Key Laboratory of Ministry of Education

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2010年第1期42-47,共6页华中科技大学学报（医学英德文版）

基　　金：supported by grants from National Basic Research Program of China(973 Program,No.2007CB935803);National Natural Sciences Foundation of China(No.30825018)

摘　　要：The binding function of EGF1 domain peptide with tissue factor(TF)and its ability of triggering coagulation were explored.The TF expression model in vitro was established by lipopolysaccha-ride induction.The affinity of EGFP-EGF1 and TF expressing cells was analyzed by fluorescence microscopy and flow cytometry(FCM).The affinity of EGFP-EGF1 and rat soluble TF was quantitated by surface plasmon resonance(SPR).The ability of EGFP-EGF1 in triggering coagulation was tested by prothrombin time assay.The FCM res...The binding function of EGF1 domain peptide with tissue factor(TF)and its ability of triggering coagulation were explored.The TF expression model in vitro was established by lipopolysaccha-ride induction.The affinity of EGFP-EGF1 and TF expressing cells was analyzed by fluorescence microscopy and flow cytometry(FCM).The affinity of EGFP-EGF1 and rat soluble TF was quantitated by surface plasmon resonance(SPR).The ability of EGFP-EGF1 in triggering coagulation was tested by prothrombin time assay.The FCM res...

关 键 词：coagulation factor Ⅶ epidermal growth factor-like domain tissue factor ANTICOAGULATION 

分 类 号：R363[医药卫生—病理学]