响应面法优化超声波辅助提取绿茶总黄酮工艺的研究  

Study on Optimization of Ultrasound-assisted Extraction Technology of Total Flavonoids from Green Tea by Response Surface Methodology

作　　者：赵熙[1] 粟本文[1] 郑红发[1] 黄怀生[1] 袁英芳[1] 

机构地区：[1]湖南省农业科学院茶叶研究所,湖南长沙410125

出　　处：《茶叶科学》2010年第4期295-301,共7页Journal of Tea Science

摘　　要：为优化超声波提取绿茶总黄酮工艺,在单因素试验基础上,采用Plackett-Burman试验设计对影响绿茶总黄酮得率的因素进行筛选,得出具有显著效应的3个因素—乙醇质量分数、液固比和提取次数。根据Box-Behnken中心组合试验设计原理进行响应面回归分析,以总黄酮得率为响应值,确定主要影响因素的最佳提取条件为乙醇质量分数77%、液固比42︰1和提取次数2次,提取率为3.76%,与模型预测值3.79%基本相符。结果表明,Plackett-Burman设计结合响应面分析法可以很好地对绿茶总黄酮提取工艺进行优化。In order to optimize the conditions for the ultrasound-extraction technology of total flavonoids from green tea,the Plackett-Burman design was introduced to evaluate the effects of five factors based on single factor test.Ethanol concentration,ratio of liquid to solid and extraction times were found to be the critical for the factors activity of total flavonoids extraction.The optimum conditions for extraction were obtained through Box-Behnken center-united experiment design and Response Surface Methodology...

关 键 词：绿茶 总黄酮 PLACKETT-BURMAN设计 响应面法 

分 类 号：TQ461[化学工程—制药化工]