Pathogenic and pathological characteristic of new type gosling viral enteritis first observed in China  

作　　者：An-Chun Cheng Ming-Shu Wang Xiao-Yue Chen Yu-Fei Guo Zhao-Yu Liu Peng-Fei Fang College of Animal Science and Veterinary Medicine,Sichuan Agricultural University,Xinkang Road,Yaan City 625014,Sichuan Province,China 

出　　处：《World Journal of Gastroenterology》2001年第5期678-684,共7页世界胃肠病学杂志（英文版）

基　　金：Supported by the Mational Mature Scientific Foundation of China,No.39970561;Funds for the Leeders in Academia of Sichuan Province,No.9900009240018.

摘　　要：AIM: To study the purifying method and characteristics of new gosling viral enteritis virus (NGVEV),the etiological agent of new gosling viral enteritis (NGVE) which was first recognized in China, as well as the pathomorphological development in goslings infected artificially with NGVEV. METHODS: (1)NGVEV virions were purified by the procedure of treatment with chloroform and ammonium sulfate precipitation, dialysis to remove the sulfate radical and ammonium ion and separation by gel filtration chromatography, and SDS-PAGE. (2)Forty-2-day-old White Sichuan goslings were orally administered with NGVEV and 24 hr later 2 birds were randomly selected and killed at 24hr intervals until death occurred. Specimens(duodenum, ileum, liver, heart, kidney, spleen, lung, proventriculus, pancreas, esophagus, and the intestinal embolus) were taken until all birds in this group died and were sectioned and stained with hemotoxylin and eosin and studied by light microscope. RESULTS: NGVEV shared the typical characteristics of Adenovirus and which structural proteins consisted of 15 polypeptides. Necrosis and sloughing of the epithelial cells covering the villus tips of the duodenum were first observed in goslings 2 days postinfection artificially with NGVEV. With the progress of infection, this lesion rapidly occurred in the epithelium at the base of the villus and with infiltration of the inflammatory cells, the jejunum tended to be involved. With the intensification of mucosa necrosis and inflammatory exudation of the small intestine, fibrinonecrotic enteritis was further developed and embolus composed of either intestinal contents wrapped by pseudo-membrane or of the mixture of fibrous exudate and necrotic intestinal mucosa were observed in the middle-lower part of the small intestine. This structure occluded the intestinal tract and made the intestine dilated in appearance. The intestinal glandular cells underwent degeneration, necrosis and might be found sloughed into the lumen. Hemorrhage and hyperemia could be observed on tAIM To study the purifying method and characteristics of neW gosling viral enteritis virus (NGVEV), the etiological agent of new gosling viral enteritis (NGVE) which was first recognized in China, as well as the pathomorphological davelapment in goslings infected artificially with NGVEV.``of treatment with chloroform and ammonium sulfate precipitation, dialysis to remove the sulfate radical and ammonium ion and separation by gel filtration Sichuan goslings were orally administered with NGVEV and 24hr later 2 birds were randomly selected and killed at 24hr intervals Until death occurred. Specimens(duodenum, ileum,liver, heart, kidney, spleen, lung, proventriculus, pancreas,esophagus, and the intestinal embolus) were taken until all birds in this group died and were sectioned and stained with hemotoxylin and ensin and studied by light microscope.``RESULTS NGVEV shared the typical characteristics of Adenovirus and which structural proteins consisted of 15polypsptides. Necrosis and sloughing of the epithelial cells covering the villus tips of the duodanum were first observed in goslings 2 days postinfectien artificially with NGVEV. With the progress of infection, this lesion rapidly occurred in the epithelium at the base of the villus and with infiltration of the inflammatory cells, the jejunum tended to be involved. With the intensification of rnucnsa necrosis and inflammatory exudation of the small intestine, fibrinenecrotic enteritis was further developed and embolus composed of either intestinal contents wrapped by pseudomembrane or of the mixture of fibrous exudate and necrotic intestinal rnucoes were observed in the middle-lower part of the small intestine. This structure occluded the intestinal tract and made the intestine dilated in appearance. The intestinal glandular cells underwent degeneration, necrosis and might be found sloughed into the lumen. Hemorrhage and hyperemia could be observed on the lung and kidney . Epithelial cells of the renal tubular underwent degeneration. In some cases, granular degener

关 键 词：ADENOVIRIDAE Animals Brain China DNA  Viral ENTERITIS Female GEESE Inclusion Bodies  Viral Poultry Diseases Research Support  Non-U.S. Gov't Virulence VISCERA 

分 类 号：R574[医药卫生—消化系统]